Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7179 | 3' | -58.4 | NC_001900.1 | + | 43336 | 0.66 | 0.526016 |
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Target: 5'- uCGGGCCGaCCGug--GGUCugCCAGAUGAa -3' miRNA: 3'- -GUCUGGC-GGCuagaCCGG--GGUCUACU- -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 21534 | 0.66 | 0.505203 |
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Target: 5'- aCAGACCGCCGA-CUu-CCUCGGcGUGAg -3' miRNA: 3'- -GUCUGGCGGCUaGAccGGGGUC-UACU- -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 30145 | 0.67 | 0.423134 |
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Target: 5'- -cGACagaGCCGAUCUGGaagauguacugaauCCCCucGGUGAu -3' miRNA: 3'- guCUGg--CGGCUAGACC--------------GGGGu-CUACU- -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 7306 | 0.68 | 0.398199 |
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Target: 5'- --cGCCGCCGGUCUGGgCUugguCGGGUGu -3' miRNA: 3'- gucUGGCGGCUAGACCgGG----GUCUACu -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 40474 | 0.69 | 0.325344 |
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Target: 5'- uGGAcCCGUCGAacccgagggugaucuUCUGGCCCCGGuccagcgGAg -3' miRNA: 3'- gUCU-GGCGGCU---------------AGACCGGGGUCua-----CU- -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 40381 | 0.7 | 0.306862 |
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Target: 5'- --cGCCGCCGuacuUCUGGggaUCCCAGAUGu -3' miRNA: 3'- gucUGGCGGCu---AGACC---GGGGUCUACu -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 21601 | 0.7 | 0.306862 |
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Target: 5'- aAGGCCGUgCGG-CUcGGCCCCAGGcUGAu -3' miRNA: 3'- gUCUGGCG-GCUaGA-CCGGGGUCU-ACU- -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 32823 | 0.7 | 0.299402 |
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Target: 5'- gGGGCCaGUCGucguugCUGGCCCCcGGUGGc -3' miRNA: 3'- gUCUGG-CGGCua----GACCGGGGuCUACU- -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 32538 | 0.71 | 0.264206 |
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Target: 5'- aGGACCGaCCGG-CUGGCCUC-GAUGu -3' miRNA: 3'- gUCUGGC-GGCUaGACCGGGGuCUACu -5' |
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| 7179 | 3' | -58.4 | NC_001900.1 | + | 36231 | 1.09 | 0.000425 |
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Target: 5'- aCAGACCGCCGAUCUGGCCCCAGAUGAg -3' miRNA: 3'- -GUCUGGCGGCUAGACCGGGGUCUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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