Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 718 | 5' | -58.1 | AC_000019.1 | + | 9253 | 0.66 | 0.433927 |
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Target: 5'- aGGCGggGGCGgAggggGCaCGcggcGACGUCGa -3' miRNA: 3'- gCCGCuuCCGCgUaa--CG-GU----CUGCGGU- -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 3272 | 0.66 | 0.395623 |
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Target: 5'- -uGCGGAGGCaaGCA-UGCCAGGuuccaGCCGg -3' miRNA: 3'- gcCGCUUCCG--CGUaACGGUCUg----CGGU- -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 9331 | 0.66 | 0.386393 |
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Target: 5'- gCGGCGgcGGCGCAUgGUUucaguGACGgCGc -3' miRNA: 3'- -GCCGCuuCCGCGUAaCGGu----CUGCgGU- -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 33030 | 0.66 | 0.386393 |
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Target: 5'- aGGCGAAGaagaGUGUGUucUGCCAGG-GCCGc -3' miRNA: 3'- gCCGCUUC----CGCGUA--ACGGUCUgCGGU- -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 15751 | 0.67 | 0.334075 |
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Target: 5'- gGGCGAAGaGC-CAUgcuuagggcgGCCAGACGUg- -3' miRNA: 3'- gCCGCUUC-CGcGUAa---------CGGUCUGCGgu -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 8438 | 0.68 | 0.317827 |
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Target: 5'- gCGGUGAcgGGGaCGCGc-GCCGGGCGgCAg -3' miRNA: 3'- -GCCGCU--UCC-GCGUaaCGGUCUGCgGU- -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 10861 | 0.81 | 0.034653 |
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Target: 5'- gGGCGAAGGCGCAccuagagUGCCAGuccuuCGCCc -3' miRNA: 3'- gCCGCUUCCGCGUa------ACGGUCu----GCGGu -5' |
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| 718 | 5' | -58.1 | AC_000019.1 | + | 15666 | 1.08 | 0.0003 |
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Target: 5'- cCGGCGAAGGCGCAUUGCCAGACGCCAc -3' miRNA: 3'- -GCCGCUUCCGCGUAACGGUCUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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