Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7180 | 5' | -57.7 | NC_001900.1 | + | 23829 | 0.66 | 0.549351 |
Target: 5'- -aUCGGCGCUGGcuUCcgGCGCUgGGg -3' miRNA: 3'- uaGGCUGCGGCCu-AGuaCGCGAgCUg -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 40256 | 0.66 | 0.548279 |
Target: 5'- cGUCCaACGUcuugagggucacgCGGGUCAUGUcCUCGACc -3' miRNA: 3'- -UAGGcUGCG-------------GCCUAGUACGcGAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 18654 | 0.66 | 0.528045 |
Target: 5'- -aCCGGCGCgGGuUCc--UGCUCGACg -3' miRNA: 3'- uaGGCUGCGgCCuAGuacGCGAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 17828 | 0.66 | 0.507053 |
Target: 5'- cUCCGAaaCGCUGG-UCAccuCGCUCGGCc -3' miRNA: 3'- uAGGCU--GCGGCCuAGUac-GCGAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 18551 | 0.67 | 0.49669 |
Target: 5'- uUCaCGGC-CCGGAUCugcaGUGCGCU-GGCa -3' miRNA: 3'- uAG-GCUGcGGCCUAG----UACGCGAgCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 38293 | 0.67 | 0.49669 |
Target: 5'- cAUCCu-CGCCuucuggcauggGGAugauUCGUGCGUUCGGCa -3' miRNA: 3'- -UAGGcuGCGG-----------CCU----AGUACGCGAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 336 | 0.67 | 0.49669 |
Target: 5'- gAUCCGGCaaccGCCGGAUCuaGCGC-CG-Cg -3' miRNA: 3'- -UAGGCUG----CGGCCUAGuaCGCGaGCuG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 5705 | 0.67 | 0.486424 |
Target: 5'- gGUCaCG-CGCCGGAUCAacgGCGggcucaccaacCUCGAUg -3' miRNA: 3'- -UAG-GCuGCGGCCUAGUa--CGC-----------GAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 16969 | 0.67 | 0.47626 |
Target: 5'- cGUUCGGCGCUGGAgCcgGUGUggucgcacUCGGCa -3' miRNA: 3'- -UAGGCUGCGGCCUaGuaCGCG--------AGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 13372 | 0.67 | 0.466202 |
Target: 5'- gAUCCGGCGUCGGAUCccaGauCUgGACg -3' miRNA: 3'- -UAGGCUGCGGCCUAGua-CgcGAgCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 27122 | 0.67 | 0.456256 |
Target: 5'- aAUCCGccagguACGCCGGAUgCGUGauCGCccCGACg -3' miRNA: 3'- -UAGGC------UGCGGCCUA-GUAC--GCGa-GCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 7390 | 0.68 | 0.426171 |
Target: 5'- gAUCCcGCGCCGGAUCGUGUuCUucaacgggaucacCGAUa -3' miRNA: 3'- -UAGGcUGCGGCCUAGUACGcGA-------------GCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 15128 | 0.68 | 0.417659 |
Target: 5'- cAUCCGGCGCaucCGGuUCucgGUGCUgGACa -3' miRNA: 3'- -UAGGCUGCG---GCCuAGua-CGCGAgCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 28835 | 0.68 | 0.390052 |
Target: 5'- -cCCGAaccaGaCCGGAgcCAUGUGCUCGAa -3' miRNA: 3'- uaGGCUg---C-GGCCUa-GUACGCGAGCUg -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 35900 | 0.68 | 0.390052 |
Target: 5'- -aCCGccaGCCGcGAUCAgGCGCUgGGCg -3' miRNA: 3'- uaGGCug-CGGC-CUAGUaCGCGAgCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 42745 | 0.69 | 0.381118 |
Target: 5'- -gCCGugGCCGGugcggC-UGCGC-CGACc -3' miRNA: 3'- uaGGCugCGGCCua---GuACGCGaGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 4173 | 0.7 | 0.3361 |
Target: 5'- -cCCGAugugcccauCGCUGGAugagauccgagaggUCGUGgGCUCGACg -3' miRNA: 3'- uaGGCU---------GCGGCCU--------------AGUACgCGAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 21586 | 0.7 | 0.307045 |
Target: 5'- -gCCGACgGCCGGcUCAaggccgUGCGgCUCGGCc -3' miRNA: 3'- uaGGCUG-CGGCCuAGU------ACGC-GAGCUG- -5' |
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7180 | 5' | -57.7 | NC_001900.1 | + | 35702 | 1.06 | 0.000716 |
Target: 5'- gAUCCGACGCCGGAUCAUGCGCUCGACc -3' miRNA: 3'- -UAGGCUGCGGCCUAGUACGCGAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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