Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7181 | 5' | -56 | NC_001900.1 | + | 45903 | 0.67 | 0.596739 |
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Target: 5'- gGUCCGCUcaagacuUCGaUGGcCGCGaggaucUCGCGGu -3' miRNA: 3'- -UAGGCGA-------AGC-ACCaGCGUgu----AGCGCC- -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 37236 | 0.67 | 0.608807 |
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Target: 5'- -gCCgGCggUCGUGGUUGC-CuUCGUGGa -3' miRNA: 3'- uaGG-CGa-AGCACCAGCGuGuAGCGCC- -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 29494 | 0.66 | 0.619799 |
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Target: 5'- aAUCCgGCUUCGUGGagGCGaAUCaGCGc -3' miRNA: 3'- -UAGG-CGAAGCACCagCGUgUAG-CGCc -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 7827 | 0.66 | 0.652801 |
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Target: 5'- -cCCGCagCGUGGaUCaC-CGUCGCGGc -3' miRNA: 3'- uaGGCGaaGCACC-AGcGuGUAGCGCC- -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 43382 | 0.67 | 0.586892 |
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Target: 5'- -aCCGUUUCGaGGUCGCgGCgGUCGCu- -3' miRNA: 3'- uaGGCGAAGCaCCAGCG-UG-UAGCGcc -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 18916 | 0.68 | 0.553247 |
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Target: 5'- gGUCCaggaggccaagaaGCUcgggaaGUGG-CGCACGUCGUGGg -3' miRNA: 3'- -UAGG-------------CGAag----CACCaGCGUGUAGCGCC- -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 25144 | 0.68 | 0.532908 |
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Target: 5'- -aCCGCUggggUCGUuGUCGUugAUCGUGa -3' miRNA: 3'- uaGGCGA----AGCAcCAGCGugUAGCGCc -5' |
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| 7181 | 5' | -56 | NC_001900.1 | + | 35342 | 1.08 | 0.000964 |
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Target: 5'- gAUCCGCUUCGUGGUCGCACAUCGCGGc -3' miRNA: 3'- -UAGGCGAAGCACCAGCGUGUAGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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