Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7182 | 3' | -58.3 | NC_001900.1 | + | 20336 | 0.66 | 0.582024 |
Target: 5'- uGUCGUgGUGACaGAC-CgGGCCg-- -3' miRNA: 3'- gCAGCAgCGCUGcCUGuGgCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 11377 | 0.66 | 0.560854 |
Target: 5'- uGUCGagggcgaaccUCGCcaGugGGACACCuuGGCCg-- -3' miRNA: 3'- gCAGC----------AGCG--CugCCUGUGG--CCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 23800 | 0.66 | 0.539901 |
Target: 5'- uGUgGUcCGaCGACGGGCuCCGGUCg-- -3' miRNA: 3'- gCAgCA-GC-GCUGCCUGuGGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 17843 | 0.67 | 0.508995 |
Target: 5'- -uUCGUCGCuGACGG-CA-CGGCCa-- -3' miRNA: 3'- gcAGCAGCG-CUGCCuGUgGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 43388 | 0.67 | 0.498856 |
Target: 5'- uCGagGUCGCGGCGGuCGCUGaguGCgUUGa -3' miRNA: 3'- -GCagCAGCGCUGCCuGUGGC---CGgAAC- -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 40632 | 0.67 | 0.478857 |
Target: 5'- uCGaUGUUGCGGaGGACGCCaGCCUUc -3' miRNA: 3'- -GCaGCAGCGCUgCCUGUGGcCGGAAc -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 27015 | 0.67 | 0.478857 |
Target: 5'- -cUCGUCGCGACGauGGCgACCguGGCCcUGa -3' miRNA: 3'- gcAGCAGCGCUGC--CUG-UGG--CCGGaAC- -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 48169 | 0.67 | 0.468027 |
Target: 5'- uGUCGaagccauUCGCGAauguuggguCGGGCugCGGCCc-- -3' miRNA: 3'- gCAGC-------AGCGCU---------GCCUGugGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 25202 | 0.68 | 0.449623 |
Target: 5'- cCGagGUUGCGguuACGGGC-CUGGCCUUc -3' miRNA: 3'- -GCagCAGCGC---UGCCUGuGGCCGGAAc -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 36302 | 0.68 | 0.449623 |
Target: 5'- aCGUgGUCGCugaggauggucgGACGGGCGacgauCCGGCCc-- -3' miRNA: 3'- -GCAgCAGCG------------CUGCCUGU-----GGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 25668 | 0.68 | 0.430689 |
Target: 5'- -aUCGcCGCGACGuGAggcggcacgguCACCGGCCg-- -3' miRNA: 3'- gcAGCaGCGCUGC-CU-----------GUGGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 26900 | 0.68 | 0.412232 |
Target: 5'- cCGUCGcCGuCGGCGGu--CUGGCCUUc -3' miRNA: 3'- -GCAGCaGC-GCUGCCuguGGCCGGAAc -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 23445 | 0.68 | 0.412232 |
Target: 5'- -uUCGUC-CGuACGGAC-CCGGCCg-- -3' miRNA: 3'- gcAGCAGcGC-UGCCUGuGGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 10489 | 0.68 | 0.412232 |
Target: 5'- aGagGcCGCGAUGGGCcUCGGCCUg- -3' miRNA: 3'- gCagCaGCGCUGCCUGuGGCCGGAac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 4621 | 0.68 | 0.412232 |
Target: 5'- uCG-CGUgGCGGgGGACucCCGGCCa-- -3' miRNA: 3'- -GCaGCAgCGCUgCCUGu-GGCCGGaac -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 46360 | 0.73 | 0.204012 |
Target: 5'- aGUCGUCGCGAagccucUGGcCGCUGGCgUUGu -3' miRNA: 3'- gCAGCAGCGCU------GCCuGUGGCCGgAAC- -5' |
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7182 | 3' | -58.3 | NC_001900.1 | + | 33644 | 1.08 | 0.000689 |
Target: 5'- gCGUCGUCGCGACGGACACCGGCCUUGu -3' miRNA: 3'- -GCAGCAGCGCUGCCUGUGGCCGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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