Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7182 | 5' | -55 | NC_001900.1 | + | 33681 | 1.11 | 0.000741 |
Target: 5'- gGCCAAGGCGCAUGUCGCCGUCUUCGAu -3' miRNA: 3'- -CGGUUCCGCGUACAGCGGCAGAAGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 43919 | 0.74 | 0.293739 |
Target: 5'- uCC-AGGCGCGUG-CGCCGUCguaGAg -3' miRNA: 3'- cGGuUCCGCGUACaGCGGCAGaagCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 23321 | 0.7 | 0.480572 |
Target: 5'- cGCCGGGGCGCGgccCGC-GUCggCGAg -3' miRNA: 3'- -CGGUUCCGCGUacaGCGgCAGaaGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 19816 | 0.69 | 0.533082 |
Target: 5'- aGCCAAGaGgGUGgcucGUCGCCGUCggaugaccUUCGAc -3' miRNA: 3'- -CGGUUC-CgCGUa---CAGCGGCAG--------AAGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 40001 | 0.68 | 0.554668 |
Target: 5'- cGCCGguaucucgcAGGUGCAUGgccucguaGCCGUCgcCGAc -3' miRNA: 3'- -CGGU---------UCCGCGUACag------CGGCAGaaGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 15431 | 0.68 | 0.576506 |
Target: 5'- cGCCuacgAGGGCGUggAUGUCGCCGccgaUCUgcaGAg -3' miRNA: 3'- -CGG----UUCCGCG--UACAGCGGC----AGAag-CU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 38813 | 0.68 | 0.587498 |
Target: 5'- gGCC-AGGCGCA--UCGCC-UCUUCc- -3' miRNA: 3'- -CGGuUCCGCGUacAGCGGcAGAAGcu -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 12985 | 0.68 | 0.598528 |
Target: 5'- cGCCGAGGCGUucGUCGCUcugaccaacgugGUCagCGGc -3' miRNA: 3'- -CGGUUCCGCGuaCAGCGG------------CAGaaGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 9596 | 0.68 | 0.598528 |
Target: 5'- cCCGAcGCGCAUGUaCGCCGagaUCGAu -3' miRNA: 3'- cGGUUcCGCGUACA-GCGGCagaAGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 34776 | 0.67 | 0.650603 |
Target: 5'- gGCCAGguugguucgagcacGGCGgGUGaCGCCGUC-UCGc -3' miRNA: 3'- -CGGUU--------------CCGCgUACaGCGGCAGaAGCu -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 11241 | 0.66 | 0.676013 |
Target: 5'- -gCAGGGUGCGacugccUGguggUGCCuGUCUUCGAc -3' miRNA: 3'- cgGUUCCGCGU------ACa---GCGG-CAGAAGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 45613 | 0.66 | 0.686999 |
Target: 5'- gGCCGGGGUGUcgGU-GCCGUUgaggUUCa- -3' miRNA: 3'- -CGGUUCCGCGuaCAgCGGCAG----AAGcu -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 28280 | 0.66 | 0.697932 |
Target: 5'- gGUCAucgaacGGGUGCggGUCGCgGUCcUUGAu -3' miRNA: 3'- -CGGU------UCCGCGuaCAGCGgCAGaAGCU- -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 2011 | 0.66 | 0.697932 |
Target: 5'- aGCguGGGCGCGUGUCauCCGagaCUUCa- -3' miRNA: 3'- -CGguUCCGCGUACAGc-GGCa--GAAGcu -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 34870 | 0.66 | 0.708799 |
Target: 5'- gGCCcaGAGGC-CGUGcCGCCGaacgUCUUCa- -3' miRNA: 3'- -CGG--UUCCGcGUACaGCGGC----AGAAGcu -5' |
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7182 | 5' | -55 | NC_001900.1 | + | 14004 | 0.66 | 0.7088 |
Target: 5'- gGCCGAGGCGgGucaguUGUCgaGCCG-CUaCGAc -3' miRNA: 3'- -CGGUUCCGCgU-----ACAG--CGGCaGAaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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