miRNA: 3'- gCGCUACUUGUcuaGUGGCuaGGGCa -5'
miRNA: 3'- -GCGCUACu---UGUCuaGUG-GCUAGGGCA- -5'
miRNA: 3'- gCGcUACU---UGUCUAGuGGCUAgGGCa -5'
miRNA: 3'- -GCGCUACu------UGUCuaGUGGCUaG-GGCA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7184 | 3' | -52.7 | NC_001900.1 | + | 24255 | 0.66 | 0.80566 |
|
Target: 5'- aGCGAUGAGCGugcCACCG--CCCGa -3' miRNA: 3'- gCGCUACUUGUcuaGUGGCuaGGGCa -5' |
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| 7184 | 3' | -52.7 | NC_001900.1 | + | 26003 | 0.66 | 0.815273 |
|
Target: 5'- uCGCGGUGucccGCAGc-CACuCGAUCUCGUc -3' miRNA: 3'- -GCGCUACu---UGUCuaGUG-GCUAGGGCA- -5' |
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| 7184 | 3' | -52.7 | NC_001900.1 | + | 15012 | 0.66 | 0.824685 |
|
Target: 5'- aGCcAUGAguuGCGGAUC-CCGGUgCCGg -3' miRNA: 3'- gCGcUACU---UGUCUAGuGGCUAgGGCa -5' |
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| 7184 | 3' | -52.7 | NC_001900.1 | + | 9190 | 0.66 | 0.824685 |
|
Target: 5'- aCGCGAUGuaccgccACGGcgUACCGAcCgCCGUg -3' miRNA: 3'- -GCGCUACu------UGUCuaGUGGCUaG-GGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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