Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7186 | 3' | -55.1 | NC_001900.1 | + | 16982 | 0.68 | 0.552244 |
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Target: 5'- -aGCCGgugUGGUCGcACUCggcaUGGACGGCa -3' miRNA: 3'- uaCGGCa--ACUAGUcUGGG----ACCUGCUG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 31973 | 0.66 | 0.675124 |
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Target: 5'- cGUGCCGUcuucgaacgcgaUGAUCAGgacACCCUcuugggugucgaGGAuCGGCu -3' miRNA: 3'- -UACGGCA------------ACUAGUC---UGGGA------------CCU-GCUG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 18004 | 0.67 | 0.585467 |
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Target: 5'- -cGUCGUUGAacaccCAGAggaUCUGGACGGCu -3' miRNA: 3'- uaCGGCAACUa----GUCUg--GGACCUGCUG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 26149 | 0.69 | 0.456869 |
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Target: 5'- -aGaCCGUUGAcCAGAgCCUGGGgGAUc -3' miRNA: 3'- uaC-GGCAACUaGUCUgGGACCUgCUG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 10901 | 0.7 | 0.446806 |
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Target: 5'- cGUGCCaccgGAUCcagcAGuuCCUGGACGACg -3' miRNA: 3'- -UACGGcaa-CUAG----UCugGGACCUGCUG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 35906 | 0.71 | 0.353534 |
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Target: 5'- -aGCCGc-GAUCAGGCgCUGGGCGuCu -3' miRNA: 3'- uaCGGCaaCUAGUCUGgGACCUGCuG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 32208 | 0.73 | 0.296911 |
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Target: 5'- -cGCUGUgcgGGUCAGAUCCaGGaACGACu -3' miRNA: 3'- uaCGGCAa--CUAGUCUGGGaCC-UGCUG- -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 34151 | 0.76 | 0.178961 |
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Target: 5'- -cGCCGagccUGAUCAGGCCCUGGAUu-- -3' miRNA: 3'- uaCGGCa---ACUAGUCUGGGACCUGcug -5' |
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| 7186 | 3' | -55.1 | NC_001900.1 | + | 30773 | 1.08 | 0.000859 |
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Target: 5'- gAUGCCGUUGAUCAGACCCUGGACGACg -3' miRNA: 3'- -UACGGCAACUAGUCUGGGACCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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