Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7186 | 5' | -56.3 | NC_001900.1 | + | 37448 | 0.66 | 0.645191 |
Target: 5'- cCGAccaCAGCCuuGAGCUgcuUGCGgUCAUCGa -3' miRNA: 3'- -GCUa--GUCGGucCUCGG---ACGC-AGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 21075 | 0.66 | 0.612094 |
Target: 5'- uGAUCuucgugcccGCCGGGGGCg-GCGUCAaCGu -3' miRNA: 3'- gCUAGu--------CGGUCCUCGgaCGCAGUaGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 43911 | 0.66 | 0.601084 |
Target: 5'- gCGGUCGuuCCAGGcGCgUGCGcCGUCGu -3' miRNA: 3'- -GCUAGUc-GGUCCuCGgACGCaGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 42222 | 0.66 | 0.601084 |
Target: 5'- aCGAUCAGCgCAGGgaAGuCCUcguagagcGCGUCGUaCGc -3' miRNA: 3'- -GCUAGUCG-GUCC--UC-GGA--------CGCAGUA-GC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 33723 | 0.66 | 0.601084 |
Target: 5'- gGAaC-GCCAGGAGCUggugcaGCGUCAgcUCGu -3' miRNA: 3'- gCUaGuCGGUCCUCGGa-----CGCAGU--AGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 40585 | 0.66 | 0.590101 |
Target: 5'- gCGGUgaCAGCCAGGAGuCCU-CGgccgCGUUGa -3' miRNA: 3'- -GCUA--GUCGGUCCUC-GGAcGCa---GUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 27458 | 0.66 | 0.586813 |
Target: 5'- gGAUCAGCCuGGGGCCgagccGCacggccuugagccgGcCGUCGg -3' miRNA: 3'- gCUAGUCGGuCCUCGGa----CG--------------CaGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 39890 | 0.67 | 0.561732 |
Target: 5'- gGAUCAGCgAucuccuccuguccggGGAGCggGCuGUCAUCGg -3' miRNA: 3'- gCUAGUCGgU---------------CCUCGgaCG-CAGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 20365 | 0.68 | 0.525234 |
Target: 5'- uCGA-CGGCCAGGAGC-UGCccugGUaCAUCGc -3' miRNA: 3'- -GCUaGUCGGUCCUCGgACG----CA-GUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 16968 | 0.68 | 0.525234 |
Target: 5'- gCGuUCGGCgCuGGAGCCgGUGUgGUCGc -3' miRNA: 3'- -GCuAGUCG-GuCCUCGGaCGCAgUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 25256 | 0.68 | 0.525234 |
Target: 5'- cCGA-UAGCCGaccGGAGCC--CGUCGUCGg -3' miRNA: 3'- -GCUaGUCGGU---CCUCGGacGCAGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 25643 | 0.68 | 0.473324 |
Target: 5'- cCGGUCAGCCAuGuGCUcGCGgaUCAUCGc -3' miRNA: 3'- -GCUAGUCGGUcCuCGGaCGC--AGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 31352 | 0.69 | 0.463249 |
Target: 5'- aCGGUCAGCaCAGGccccagAGCCUGCagagcgcccGUCAgcaUCGu -3' miRNA: 3'- -GCUAGUCG-GUCC------UCGGACG---------CAGU---AGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 8637 | 0.71 | 0.352159 |
Target: 5'- uGGUCGgcuGCCGGGucuccgacGGCCUGCuguucGUCAUCGa -3' miRNA: 3'- gCUAGU---CGGUCC--------UCGGACG-----CAGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 5741 | 0.71 | 0.343794 |
Target: 5'- aCGGUCGGCCcgacgugaGGGAGCCaGCGUgagcaaCGUCu -3' miRNA: 3'- -GCUAGUCGG--------UCCUCGGaCGCA------GUAGc -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 29802 | 0.72 | 0.311777 |
Target: 5'- gCGA-CAGCCAGGAccguggucccuGCUUcGCGUCGUUGa -3' miRNA: 3'- -GCUaGUCGGUCCU-----------CGGA-CGCAGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 35553 | 0.73 | 0.268086 |
Target: 5'- aGAUCAGCCuGGAGCUgguagGUGUacgcccCGUCGg -3' miRNA: 3'- gCUAGUCGGuCCUCGGa----CGCA------GUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 42468 | 0.75 | 0.190473 |
Target: 5'- uGAaCAGCCAGG-GCUUGC-UCAUCGg -3' miRNA: 3'- gCUaGUCGGUCCuCGGACGcAGUAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 28141 | 0.75 | 0.185401 |
Target: 5'- cCGGUgAGCCAGGAGCCgccgaagGCgGUC-UCGg -3' miRNA: 3'- -GCUAgUCGGUCCUCGGa------CG-CAGuAGC- -5' |
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7186 | 5' | -56.3 | NC_001900.1 | + | 30813 | 1.09 | 0.000666 |
Target: 5'- uCGAUCAGCCAGGAGCCUGCGUCAUCGa -3' miRNA: 3'- -GCUAGUCGGUCCUCGGACGCAGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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