Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7187 | 5' | -52.8 | NC_001900.1 | + | 25530 | 0.73 | 0.382982 |
Target: 5'- cGACCGAUCUUGGCGua-GCCCUUc-- -3' miRNA: 3'- -CUGGCUAGAGUCGCuugUGGGAAcug -5' |
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7187 | 5' | -52.8 | NC_001900.1 | + | 9191 | 0.74 | 0.347406 |
Target: 5'- cGCUGAUC-CGGCGAuCGCCCgaGACg -3' miRNA: 3'- cUGGCUAGaGUCGCUuGUGGGaaCUG- -5' |
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7187 | 5' | -52.8 | NC_001900.1 | + | 29404 | 0.78 | 0.200111 |
Target: 5'- cGAgCGAUCUC-GCGAGCGCCCcagUUGGCc -3' miRNA: 3'- -CUgGCUAGAGuCGCUUGUGGG---AACUG- -5' |
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7187 | 5' | -52.8 | NC_001900.1 | + | 30654 | 1.1 | 0.001177 |
Target: 5'- uGACCGAUCUCAGCGAACACCCUUGACg -3' miRNA: 3'- -CUGGCUAGAGUCGCUUGUGGGAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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