Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7188 | 5' | -56.9 | NC_001900.1 | + | 516 | 0.67 | 0.473034 |
Target: 5'- -gUGCCCuacGUGCCcuUUACaGCCACcCGGAc -3' miRNA: 3'- uuGCGGG---UACGG--AAUG-CGGUGuGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 4362 | 0.69 | 0.377161 |
Target: 5'- cAGCGgCCAgcgGCCUgGCuGCCGCAgGGGa -3' miRNA: 3'- -UUGCgGGUa--CGGAaUG-CGGUGUgCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 13160 | 0.68 | 0.433141 |
Target: 5'- uACGCCCAgcGCCUgaucGCGgCugGCGGu -3' miRNA: 3'- uUGCGGGUa-CGGAa---UGCgGugUGCCu -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 15831 | 0.75 | 0.155804 |
Target: 5'- cGugGCUgGUGCCgaagUACGCCAgcguCACGGAg -3' miRNA: 3'- -UugCGGgUACGGa---AUGCGGU----GUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 15963 | 0.67 | 0.493663 |
Target: 5'- --aGCCCAgaaGCUgauCGCCGCGCuGGAc -3' miRNA: 3'- uugCGGGUa--CGGaauGCGGUGUG-CCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 24470 | 0.66 | 0.53608 |
Target: 5'- --aGCCCAUGCCUUGuccaccacCGCCAgCuccgaGGAg -3' miRNA: 3'- uugCGGGUACGGAAU--------GCGGU-Gug---CCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 25939 | 0.66 | 0.556677 |
Target: 5'- cGCGCUCAgcggUGCCUUcUGCUcgauccgGCACGGGu -3' miRNA: 3'- uUGCGGGU----ACGGAAuGCGG-------UGUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 27110 | 0.67 | 0.504131 |
Target: 5'- cGugGCCCAcGCCca--GCCuCACGGGc -3' miRNA: 3'- -UugCGGGUaCGGaaugCGGuGUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 29770 | 1.07 | 0.000733 |
Target: 5'- gAACGCCCAUGCCUUACGCCACACGGAg -3' miRNA: 3'- -UUGCGGGUACGGAAUGCGGUGUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 30378 | 0.69 | 0.363078 |
Target: 5'- uGCGCCUugaucuggucgagcuGcGCCUgcaGCGCCGCACGGc -3' miRNA: 3'- uUGCGGG---------------UaCGGAa--UGCGGUGUGCCu -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 32077 | 0.73 | 0.227888 |
Target: 5'- -cCGUCCAUGCCgagUGCGaCCACACcGGc -3' miRNA: 3'- uuGCGGGUACGGa--AUGC-GGUGUG-CCu -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 35640 | 0.67 | 0.493663 |
Target: 5'- cGACGCCCuucuUGCCccggaaccaGCGCCAUgugccucugGCGGGg -3' miRNA: 3'- -UUGCGGGu---ACGGaa-------UGCGGUG---------UGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 38644 | 0.73 | 0.198819 |
Target: 5'- --aGCCCAUGuCCUUGCGagcccgcUCACGCGGGa -3' miRNA: 3'- uugCGGGUAC-GGAAUGC-------GGUGUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 40200 | 0.69 | 0.40454 |
Target: 5'- -cCGCgCAUGUCgaACGCCAC-CGGGu -3' miRNA: 3'- uuGCGgGUACGGaaUGCGGUGuGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 40439 | 0.69 | 0.395275 |
Target: 5'- gGGCGCCgGUGCCUacCGUUgagaccccgACGCGGAa -3' miRNA: 3'- -UUGCGGgUACGGAauGCGG---------UGUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 43095 | 0.66 | 0.578597 |
Target: 5'- gGGCGugcCCCAUGCCgu-CGCCGuucuugaucagcuCAUGGAg -3' miRNA: 3'- -UUGC---GGGUACGGaauGCGGU-------------GUGCCU- -5' |
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7188 | 5' | -56.9 | NC_001900.1 | + | 47344 | 0.68 | 0.423476 |
Target: 5'- cAUGCUCAUGCCcuugggcuugACGCCgucgaacuccaGCGCGGAg -3' miRNA: 3'- uUGCGGGUACGGaa--------UGCGG-----------UGUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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