Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7189 | 3' | -52.9 | NC_001900.1 | + | 20866 | 0.66 | 0.801625 |
Target: 5'- cGGGAUG-GCUGCGugGucuggGACa-UCGAg -3' miRNA: 3'- aCCCUACuCGACGUugU-----CUGgaAGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 4617 | 0.66 | 0.801625 |
Target: 5'- cGGGAucUGA-CUGUAAauCAGACgCUUCGGc -3' miRNA: 3'- aCCCU--ACUcGACGUU--GUCUG-GAAGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 195 | 0.67 | 0.739027 |
Target: 5'- gUGGGGUG-GCUGuCAaacGCAGAcuCCUUCc- -3' miRNA: 3'- -ACCCUACuCGAC-GU---UGUCU--GGAAGcu -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 42702 | 0.67 | 0.717102 |
Target: 5'- gGGGAUGAcGUUGUugaagcucACAGACCcuccUCGAu -3' miRNA: 3'- aCCCUACU-CGACGu-------UGUCUGGa---AGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 16012 | 0.68 | 0.638183 |
Target: 5'- cGGGccGAGCUGUAcgcgaccguacuGCGGACCUg-GAu -3' miRNA: 3'- aCCCuaCUCGACGU------------UGUCUGGAagCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 48645 | 0.75 | 0.291035 |
Target: 5'- aUGGGugcuGUGAGCUGCAcaaucGCGGGCUgUCGGg -3' miRNA: 3'- -ACCC----UACUCGACGU-----UGUCUGGaAGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 28226 | 1.09 | 0.001305 |
Target: 5'- gUGGGAUGAGCUGCAACAGACCUUCGAc -3' miRNA: 3'- -ACCCUACUCGACGUUGUCUGGAAGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 45742 | 0.81 | 0.123406 |
Target: 5'- gGGGGUGGGCUGCuuGguGACCgggUCGAg -3' miRNA: 3'- aCCCUACUCGACGu-UguCUGGa--AGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 24137 | 0.7 | 0.547766 |
Target: 5'- uUGGGGUGAGCUGCc-UAGcGCCgccgUCGu -3' miRNA: 3'- -ACCCUACUCGACGuuGUC-UGGa---AGCu -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 18194 | 0.69 | 0.592693 |
Target: 5'- cGGGAgGAGgUGCAugAGGCgcUCGAc -3' miRNA: 3'- aCCCUaCUCgACGUugUCUGgaAGCU- -5' |
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7189 | 3' | -52.9 | NC_001900.1 | + | 20538 | 0.67 | 0.728116 |
Target: 5'- cUGGaGAUGcGCgUGCAGCAGcgaGCgUUCGAc -3' miRNA: 3'- -ACC-CUACuCG-ACGUUGUC---UGgAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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