Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7190 | 5' | -57.8 | NC_001900.1 | + | 40299 | 0.66 | 0.57224 |
Target: 5'- -gUGGCCGgucggcagugagccgCUgCGGCCGuGGUUGGCc -3' miRNA: 3'- ggACCGGCa--------------GAgGUCGGU-CCAACUGc -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 38366 | 0.67 | 0.518372 |
Target: 5'- aCCgGGCuCGUCgccgUCAGCCAGGaUGuucACGu -3' miRNA: 3'- -GGaCCG-GCAGa---GGUCGGUCCaAC---UGC- -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 2633 | 0.67 | 0.508035 |
Target: 5'- cCCUGGgCGUCguuaagCgGGCCAGGUgccuGCu -3' miRNA: 3'- -GGACCgGCAGa-----GgUCGGUCCAac--UGc -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 28712 | 0.67 | 0.477573 |
Target: 5'- uCCUGGCCGUCgaagaagaCGGCCcGGUcUGuCa -3' miRNA: 3'- -GGACCGGCAGag------GUCGGuCCA-ACuGc -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 20350 | 0.67 | 0.46762 |
Target: 5'- aCCgGGCCGUCUUCuucgacGGCCAGGa----- -3' miRNA: 3'- -GGaCCGGCAGAGG------UCGGUCCaacugc -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 34755 | 0.68 | 0.448041 |
Target: 5'- aCgGGUCGUCuuguucgcUCUGGCCAGGUUGGu- -3' miRNA: 3'- gGaCCGGCAG--------AGGUCGGUCCAACUgc -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 32050 | 0.69 | 0.410298 |
Target: 5'- --cGGCCGUU---GGCgAGGUUGACGa -3' miRNA: 3'- ggaCCGGCAGaggUCGgUCCAACUGC- -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 19689 | 0.69 | 0.410298 |
Target: 5'- cCCUGGCCG-UUCCcGCCGGGagu-CGa -3' miRNA: 3'- -GGACCGGCaGAGGuCGGUCCaacuGC- -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 20968 | 0.69 | 0.374596 |
Target: 5'- aCCUGGCUGgagaCGGCCAGGUcGAa- -3' miRNA: 3'- -GGACCGGCagagGUCGGUCCAaCUgc -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 29278 | 0.69 | 0.366005 |
Target: 5'- cCCcGG-CGUCUUgAGCCGgcGGUUGGCGa -3' miRNA: 3'- -GGaCCgGCAGAGgUCGGU--CCAACUGC- -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 31014 | 0.7 | 0.341057 |
Target: 5'- --cGGCCG----CAGCCAGGUUGAUGa -3' miRNA: 3'- ggaCCGGCagagGUCGGUCCAACUGC- -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 33965 | 0.72 | 0.247428 |
Target: 5'- aCCa-GCCGguggCUCCAGuCCAGGUcGACGg -3' miRNA: 3'- -GGacCGGCa---GAGGUC-GGUCCAaCUGC- -5' |
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7190 | 5' | -57.8 | NC_001900.1 | + | 28099 | 1.11 | 0.000373 |
Target: 5'- aCCUGGCCGUCUCCAGCCAGGUUGACGa -3' miRNA: 3'- -GGACCGGCAGAGGUCGGUCCAACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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