Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7193 | 3' | -54.7 | NC_001900.1 | + | 23670 | 0.69 | 0.506009 |
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Target: 5'- -gGCUCUGACCAcUUCGUCGGcucgGCGu -3' miRNA: 3'- caCGAGACUGGUuAGGCGGUCca--UGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 36859 | 0.68 | 0.560108 |
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Target: 5'- -cGCUCUG-CCAGUUgGCCguugcacccuugAGGUACu -3' miRNA: 3'- caCGAGACuGGUUAGgCGG------------UCCAUGc -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 8632 | 0.68 | 0.604501 |
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Target: 5'- -gGCUCUGGUCGG-CUGCCGGGUcuccgACGg -3' miRNA: 3'- caCGAGACUGGUUaGGCGGUCCA-----UGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 3547 | 0.67 | 0.626881 |
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Target: 5'- -cGCUCggGGCCGGUaaCgGCCuGGUGCa -3' miRNA: 3'- caCGAGa-CUGGUUA--GgCGGuCCAUGc -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 13000 | 0.67 | 0.626881 |
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Target: 5'- -cGCUCUGACCAAcgUgGUCAGcGgcgGCGg -3' miRNA: 3'- caCGAGACUGGUUa-GgCGGUC-Ca--UGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 13662 | 0.67 | 0.638084 |
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Target: 5'- -gGCUCcGAUCAAguacCCGCCGGGguuucuccugGCGg -3' miRNA: 3'- caCGAGaCUGGUUa---GGCGGUCCa---------UGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 42387 | 0.67 | 0.64928 |
|
Target: 5'- -cGCUCgagcGACC-AUCCGCuCAGG-AUGg -3' miRNA: 3'- caCGAGa---CUGGuUAGGCG-GUCCaUGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 30928 | 0.67 | 0.660461 |
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Target: 5'- cUGCgCcGACgAGUCUGCCAGG-ACGc -3' miRNA: 3'- cACGaGaCUGgUUAGGCGGUCCaUGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 36559 | 0.67 | 0.64928 |
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Target: 5'- gGUGgUCUGACCGAUgUagGUCGGGaACGg -3' miRNA: 3'- -CACgAGACUGGUUAgG--CGGUCCaUGC- -5' |
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| 7193 | 3' | -54.7 | NC_001900.1 | + | 27110 | 1.08 | 0.001074 |
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Target: 5'- aGUGCUCUGACCAAUCCGCCAGGUACGc -3' miRNA: 3'- -CACGAGACUGGUUAGGCGGUCCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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