Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7193 | 5' | -56.5 | NC_001900.1 | + | 20531 | 0.69 | 0.439154 |
Target: 5'- gGUUGCCCUGgagauGCGCgUGCAG-CAGCGa -3' miRNA: 3'- -UAGCGGGGC-----UGCGgACGUUaGUUGCc -5' |
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7193 | 5' | -56.5 | NC_001900.1 | + | 45532 | 0.7 | 0.374358 |
Target: 5'- --gGCCCCGAgCGCC-GC-GUCGAUGGc -3' miRNA: 3'- uagCGGGGCU-GCGGaCGuUAGUUGCC- -5' |
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7193 | 5' | -56.5 | NC_001900.1 | + | 15419 | 0.66 | 0.639898 |
Target: 5'- cGUCGaCCCgGACGCCUaCGAgggCGugGa -3' miRNA: 3'- -UAGC-GGGgCUGCGGAcGUUa--GUugCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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