Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7194 | 3' | -51 | NC_001900.1 | + | 34559 | 0.66 | 0.912031 |
Target: 5'- aGACGGUGccGCCG-AUGG-CgGCUCGGg -3' miRNA: 3'- cUUGUCGC--UGGCuUACUaGaCGAGCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 29206 | 0.66 | 0.912031 |
Target: 5'- gGAGCc-CGACuCGGAgcUGAUCUGCUCc- -3' miRNA: 3'- -CUUGucGCUG-GCUU--ACUAGACGAGcc -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 13354 | 0.66 | 0.912031 |
Target: 5'- cGAACAGguCGAgCGcAUGAUCcgGCgUCGGa -3' miRNA: 3'- -CUUGUC--GCUgGCuUACUAGa-CG-AGCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 42824 | 0.66 | 0.912031 |
Target: 5'- -cAUGGuCGACCGAAUGAUCcgggagUGCgugUGGc -3' miRNA: 3'- cuUGUC-GCUGGCUUACUAG------ACGa--GCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 44801 | 0.67 | 0.850097 |
Target: 5'- -cGCAGCGGaaGcuUGAUCUGCgucaCGGc -3' miRNA: 3'- cuUGUCGCUggCuuACUAGACGa---GCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 36122 | 0.68 | 0.793245 |
Target: 5'- cAGCGGUGAUCGcGAUGAUguugacgUGCUCGGu -3' miRNA: 3'- cUUGUCGCUGGC-UUACUAg------ACGAGCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 41850 | 0.69 | 0.740836 |
Target: 5'- --uCAGUGAUCGAAgucguUGAUCUGCaUGGu -3' miRNA: 3'- cuuGUCGCUGGCUU-----ACUAGACGaGCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 47711 | 0.7 | 0.729951 |
Target: 5'- cGGGCGGCGACCGccagcgcguUGAacgccgcacgcuUCUGCgCGGg -3' miRNA: 3'- -CUUGUCGCUGGCuu-------ACU------------AGACGaGCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 38075 | 0.74 | 0.484577 |
Target: 5'- uGACAGCG-CCG-AUGAUCUG-UCGGc -3' miRNA: 3'- cUUGUCGCuGGCuUACUAGACgAGCC- -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 42077 | 0.74 | 0.453388 |
Target: 5'- gGGGCAGCGACCGggUGgAUCcaCUCGu -3' miRNA: 3'- -CUUGUCGCUGGCuuAC-UAGacGAGCc -5' |
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7194 | 3' | -51 | NC_001900.1 | + | 26426 | 1.1 | 0.002033 |
Target: 5'- uGAACAGCGACCGAAUGAUCUGCUCGGu -3' miRNA: 3'- -CUUGUCGCUGGCUUACUAGACGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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