Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7194 | 5' | -55.9 | NC_001900.1 | + | 47817 | 0.66 | 0.685631 |
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Target: 5'- cGCCGccAGCUc----CGCUGCCGCCGc -3' miRNA: 3'- cCGGCu-UCGAcuuuaGUGGCGGCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 22155 | 0.68 | 0.522311 |
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Target: 5'- -aCUGAGGCUGAAGgccagACCGCCGaCGg -3' miRNA: 3'- ccGGCUUCGACUUUag---UGGCGGCgGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 14003 | 0.68 | 0.522311 |
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Target: 5'- uGGCCGAGGCgGGucaguuGUCGa-GCCGCUa -3' miRNA: 3'- -CCGGCUUCGaCUu-----UAGUggCGGCGGc -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 33884 | 0.68 | 0.522311 |
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Target: 5'- uGCCaGGAGCccuugaccucGGAAUCGCCGCCcucgaaGCCGa -3' miRNA: 3'- cCGG-CUUCGa---------CUUUAGUGGCGG------CGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 36029 | 0.68 | 0.532909 |
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Target: 5'- cGGUgGAugucaaguuaGGCUcagcccUCGCCGCCGCCGc -3' miRNA: 3'- -CCGgCU----------UCGAcuuu--AGUGGCGGCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 14998 | 0.68 | 0.551095 |
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Target: 5'- cGGCCGAggaguucagccaugAGUUGcGGAUC-CCGgUGCCGg -3' miRNA: 3'- -CCGGCU--------------UCGAC-UUUAGuGGCgGCGGC- -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 25199 | 0.68 | 0.554325 |
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Target: 5'- uGGCCGAGGUUGcgGUUACgGgCCugGCCu -3' miRNA: 3'- -CCGGCUUCGACuuUAGUGgC-GG--CGGc -5' |
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| 7194 | 5' | -55.9 | NC_001900.1 | + | 38577 | 0.68 | 0.554325 |
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Target: 5'- aGGCCGAGGCc-----CAUCGCgGCCu -3' miRNA: 3'- -CCGGCUUCGacuuuaGUGGCGgCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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