Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7196 | 5' | -53.5 | NC_001900.1 | + | 1996 | 0.68 | 0.699779 |
Target: 5'- gCCGACCuacgucgcagcGUGGGCGCG-UGUCauccgaGACu -3' miRNA: 3'- -GGCUGG-----------CAUCCGCGCaACAGcaa---CUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 3070 | 0.74 | 0.385735 |
Target: 5'- gCCGACCcgaccGGCGgGaagGUCGUUGACg -3' miRNA: 3'- -GGCUGGcau--CCGCgCaa-CAGCAACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 3399 | 0.67 | 0.76346 |
Target: 5'- gCCGGCCGUGaGCGUGUgcGcCGUgaaGGCa -3' miRNA: 3'- -GGCUGGCAUcCGCGCAa-CaGCAa--CUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 15778 | 0.67 | 0.746855 |
Target: 5'- gCCGGCCGa---CGCGUUGUCGaucacguucaagggUGACg -3' miRNA: 3'- -GGCUGGCauccGCGCAACAGCa-------------ACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 17887 | 0.68 | 0.664673 |
Target: 5'- gCCaGAUCGUAGGUGCGgucauucaGUUGGCc -3' miRNA: 3'- -GG-CUGGCAUCCGCGCaacag---CAACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 18068 | 0.68 | 0.721397 |
Target: 5'- gCGGCCGUGgugcaggcuGGCGCGUccaucgcGUCGUuccugaucggUGGCa -3' miRNA: 3'- gGCUGGCAU---------CCGCGCAa------CAGCA----------ACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 25142 | 0.74 | 0.367978 |
Target: 5'- -aGACCGcUGGG-GuCGUUGUCGUUGAUc -3' miRNA: 3'- ggCUGGC-AUCCgC-GCAACAGCAACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 25486 | 1.12 | 0.000925 |
Target: 5'- gCCGACCGUAGGCGCGUUGUCGUUGACg -3' miRNA: 3'- -GGCUGGCAUCCGCGCAACAGCAACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 28196 | 0.67 | 0.75312 |
Target: 5'- gCUGGCCuu--GUGCGaUGUCGUUGGCc -3' miRNA: 3'- -GGCUGGcaucCGCGCaACAGCAACUG- -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 42521 | 0.66 | 0.793614 |
Target: 5'- gCUGGCUGUAGGC-CGgUUGgaugcCGUUGAg -3' miRNA: 3'- -GGCUGGCAUCCGcGC-AACa----GCAACUg -5' |
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7196 | 5' | -53.5 | NC_001900.1 | + | 43379 | 0.67 | 0.76346 |
Target: 5'- gUGACCGUuucgAGGuCGCGgcgGUCGcUGAg -3' miRNA: 3'- gGCUGGCA----UCC-GCGCaa-CAGCaACUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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