Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7197 | 3' | -60.5 | NC_001900.1 | + | 25361 | 1.04 | 0.000644 |
Target: 5'- -aAGAUGGUCCCGGCUCCCCAGGAGCa -3' miRNA: 3'- ucUCUACCAGGGCCGAGGGGUCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 25022 | 0.73 | 0.14376 |
Target: 5'- --cGGUGGUCCCGGUgCCCguGGccaGGCg -3' miRNA: 3'- ucuCUACCAGGGCCGaGGGguCC---UCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 20743 | 0.71 | 0.187615 |
Target: 5'- aAGcuGUGG-CUCGGCUCauuccaCCGGGAGCa -3' miRNA: 3'- -UCucUACCaGGGCCGAGg-----GGUCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 26658 | 0.71 | 0.192093 |
Target: 5'- cAGAGGUGGUUuuuagcccgaggaCCGGaUCCCCgagaGGGGGCc -3' miRNA: 3'- -UCUCUACCAG-------------GGCCgAGGGG----UCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 9215 | 0.7 | 0.236749 |
Target: 5'- cGAGAUGGUCUCGGCgUUCgAGGAc- -3' miRNA: 3'- uCUCUACCAGGGCCGaGGGgUCCUcg -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 14026 | 0.69 | 0.248412 |
Target: 5'- cGAGAaGGUCUaccggaugCGGUUCCCUcgcuccuucaccaAGGAGCa -3' miRNA: 3'- uCUCUaCCAGG--------GCCGAGGGG-------------UCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 15508 | 0.69 | 0.255379 |
Target: 5'- aGGAGAgcuUCCCgcaGGCUCCCUAcGAGCg -3' miRNA: 3'- -UCUCUaccAGGG---CCGAGGGGUcCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 8696 | 0.68 | 0.296221 |
Target: 5'- aGGAGAUGcugaCCCGGCUCgCC--GAGCa -3' miRNA: 3'- -UCUCUACca--GGGCCGAGgGGucCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 22397 | 0.68 | 0.329281 |
Target: 5'- cGGGGAUccGGUCCUcgGGCUaaaaaccaccucugaCCUguGGAGCg -3' miRNA: 3'- -UCUCUA--CCAGGG--CCGA---------------GGGguCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 35787 | 0.67 | 0.358301 |
Target: 5'- -cAGGUcGUCCUGcgaccaGUUCUCCAGGAGCa -3' miRNA: 3'- ucUCUAcCAGGGC------CGAGGGGUCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 32119 | 0.67 | 0.361634 |
Target: 5'- uGGGGAU-GUCCUGGUagguguugggagcgaUCUCCcGGAGCu -3' miRNA: 3'- -UCUCUAcCAGGGCCG---------------AGGGGuCCUCG- -5' |
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7197 | 3' | -60.5 | NC_001900.1 | + | 7074 | 0.66 | 0.401487 |
Target: 5'- cGAGAUGGUCCUcacGCUCCaUCGcGAGUc -3' miRNA: 3'- uCUCUACCAGGGc--CGAGG-GGUcCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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