Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7199 | 3' | -60.7 | NC_001900.1 | + | 17594 | 0.66 | 0.388572 |
Target: 5'- cGUCCGGGggaucuaccacugaUGGCCGaucggauccagguaGUGAUCGcgcUGCCGCg -3' miRNA: 3'- -CAGGUUC--------------GCCGGC--------------CACUGGC---ACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 7166 | 0.66 | 0.430105 |
Target: 5'- -gCC-AGCGGCuucauCGGUGGCCGggacuCCGUg -3' miRNA: 3'- caGGuUCGCCG-----GCCACUGGCac---GGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 4285 | 0.66 | 0.420855 |
Target: 5'- -gCCAAGCGGgCGGUuccccaGAgCGUGgggagcccCCGCa -3' miRNA: 3'- caGGUUCGCCgGCCA------CUgGCAC--------GGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 40128 | 0.66 | 0.410819 |
Target: 5'- uUCCuggccAGCaGCCGcGUccagcagGACCGcUGCCGCg -3' miRNA: 3'- cAGGu----UCGcCGGC-CA-------CUGGC-ACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 48541 | 0.66 | 0.402719 |
Target: 5'- uGUCCGGGUGGCUguaaaGGgcacguagGGCaCGUGUgGCa -3' miRNA: 3'- -CAGGUUCGCCGG-----CCa-------CUG-GCACGgCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 27448 | 0.66 | 0.402719 |
Target: 5'- cGUUCGAcGCGGaucagcCUGG-GGCCGaGCCGCa -3' miRNA: 3'- -CAGGUU-CGCC------GGCCaCUGGCaCGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 5004 | 0.66 | 0.402719 |
Target: 5'- cUCCAgcacGGCGGCgGG-GGCCGguacUCGCa -3' miRNA: 3'- cAGGU----UCGCCGgCCaCUGGCac--GGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 14166 | 0.66 | 0.402719 |
Target: 5'- cGUCCGGGCGGCgGGUcgcaagcuGAagaCGUucgGCgGCa -3' miRNA: 3'- -CAGGUUCGCCGgCCA--------CUg--GCA---CGgCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 48537 | 0.66 | 0.385956 |
Target: 5'- uUCCAGGCcGCgaGGuUGGCCGgaacguagguugucgGCCGCa -3' miRNA: 3'- cAGGUUCGcCGg-CC-ACUGGCa--------------CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 5956 | 0.66 | 0.385087 |
Target: 5'- cUCUggGCuGGCUGcUGaACCGgccagGCCGCa -3' miRNA: 3'- cAGGuuCG-CCGGCcAC-UGGCa----CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 36585 | 0.67 | 0.359614 |
Target: 5'- --aCGGGCuGUCGGUG-CCGUgcaucGCCGCg -3' miRNA: 3'- cagGUUCGcCGGCCACuGGCA-----CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 39274 | 0.67 | 0.375609 |
Target: 5'- gGUCCGguucggaAGCGGgaccaCCGG-GACCGcGCCGa -3' miRNA: 3'- -CAGGU-------UCGCC-----GGCCaCUGGCaCGGCg -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 34785 | 0.67 | 0.367973 |
Target: 5'- gGUUCGAGCacGGCgGGUGacGCCGUcUCGCu -3' miRNA: 3'- -CAGGUUCG--CCGgCCAC--UGGCAcGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 42850 | 0.67 | 0.367973 |
Target: 5'- -cCCAGGauGCCGGgGACgagaGUGCCGa -3' miRNA: 3'- caGGUUCgcCGGCCaCUGg---CACGGCg -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 26883 | 0.67 | 0.376464 |
Target: 5'- gGUCgCAA-CGGCCGGa-ACCGUcGCCGUc -3' miRNA: 3'- -CAG-GUUcGCCGGCCacUGGCA-CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 34858 | 0.67 | 0.351389 |
Target: 5'- -gCCAgaGGCaccGGCCcagaGGCCGUGCCGCc -3' miRNA: 3'- caGGU--UCG---CCGGcca-CUGGCACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 2433 | 0.67 | 0.343298 |
Target: 5'- -gCCGGGUGGCUGuGUGAggucgacggccCCG-GCUGCg -3' miRNA: 3'- caGGUUCGCCGGC-CACU-----------GGCaCGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 15447 | 0.67 | 0.335342 |
Target: 5'- -aUCGAGCuGCCGaucgaUGACCuUGCCGCa -3' miRNA: 3'- caGGUUCGcCGGCc----ACUGGcACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 46034 | 0.68 | 0.319837 |
Target: 5'- cUCCAGGCGGaCCcGUcGCCG-GUCGCc -3' miRNA: 3'- cAGGUUCGCC-GGcCAcUGGCaCGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 6007 | 0.68 | 0.312288 |
Target: 5'- aUCgGcGCGaGCCGGUGACCu--CCGCg -3' miRNA: 3'- cAGgUuCGC-CGGCCACUGGcacGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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