Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7200 | 3' | -51.2 | NC_001900.1 | + | 18913 | 0.66 | 0.885661 |
Target: 5'- -gAUGGUCcAGGAG---GCCAAGAAGc -3' miRNA: 3'- agUGCCAGcUCCUCaugUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 22854 | 1.1 | 0.002205 |
Target: 5'- aUCACGGUCGAGGAGUACACCAAGAAGu -3' miRNA: 3'- -AGUGCCAGCUCCUCAUGUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 8352 | 0.76 | 0.379474 |
Target: 5'- gCugGGcUUGGGGGaUGCGCCAGGAAGg -3' miRNA: 3'- aGugCC-AGCUCCUcAUGUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 13821 | 0.71 | 0.610015 |
Target: 5'- gCugGGU-GAGcGGGUGgACCGAGAAGc -3' miRNA: 3'- aGugCCAgCUC-CUCAUgUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 1288 | 0.69 | 0.755147 |
Target: 5'- gCACcGUCGAcgcugagcuGGAGaaggGCACCAAGggGu -3' miRNA: 3'- aGUGcCAGCU---------CCUCa---UGUGGUUCuuC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 14996 | 0.69 | 0.765722 |
Target: 5'- -aGCGGcCGAGGAGUucaGCCAuGAGu -3' miRNA: 3'- agUGCCaGCUCCUCAug-UGGUuCUUc -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 5276 | 0.68 | 0.816159 |
Target: 5'- cUCACaG-CGAGGAGcgGCccgACCGGGAAGu -3' miRNA: 3'- -AGUGcCaGCUCCUCa-UG---UGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 48124 | 0.67 | 0.825674 |
Target: 5'- gUCGCGGuugcuugcgaUCGAGGAGUu--CCuGGAGGc -3' miRNA: 3'- -AGUGCC----------AGCUCCUCAuguGGuUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 43910 | 0.67 | 0.834972 |
Target: 5'- -aGCGGUCGuuccAGGcgcGUGCGCCGucguAGAGGa -3' miRNA: 3'- agUGCCAGC----UCCu--CAUGUGGU----UCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 8789 | 0.66 | 0.900422 |
Target: 5'- aCACGGUCGAGGAcaugacccgcGUGacccUCAAGAc- -3' miRNA: 3'- aGUGCCAGCUCCU----------CAUgu--GGUUCUuc -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 42429 | 0.71 | 0.632734 |
Target: 5'- aUCugGGUgaacgCGucGGAGUcCGCCGAGAAGu -3' miRNA: 3'- -AGugCCA-----GCu-CCUCAuGUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 23743 | 0.7 | 0.66678 |
Target: 5'- gCACGGUCGAcg---GCAUCGAGAAGg -3' miRNA: 3'- aGUGCCAGCUccucaUGUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 33909 | 0.68 | 0.786422 |
Target: 5'- aCGCGGU-GAGGAacuggucguuGUcaGCACCGAGAAc -3' miRNA: 3'- aGUGCCAgCUCCU----------CA--UGUGGUUCUUc -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 8321 | 0.67 | 0.861463 |
Target: 5'- -gACGGguUCcGGGAGgGCAUCGAGAAGc -3' miRNA: 3'- agUGCC--AGcUCCUCaUGUGGUUCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 8475 | 0.66 | 0.869794 |
Target: 5'- aCGCGGcCGAGGAcuccuggcuGU-CACCGcAGGAGu -3' miRNA: 3'- aGUGCCaGCUCCU---------CAuGUGGU-UCUUC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 6750 | 0.66 | 0.877863 |
Target: 5'- cCGuCGGUCGAGaaGGGUGuCGCCGAGcuGa -3' miRNA: 3'- aGU-GCCAGCUC--CUCAU-GUGGUUCuuC- -5' |
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7200 | 3' | -51.2 | NC_001900.1 | + | 20881 | 0.66 | 0.900422 |
Target: 5'- gUCugGGacaUCGAGGAcaacucuggcuGggGCACCGAGAc- -3' miRNA: 3'- -AGugCC---AGCUCCU-----------Ca-UGUGGUUCUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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