Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7200 | 5' | -58.5 | NC_001900.1 | + | 35218 | 0.66 | 0.494805 |
Target: 5'- cACCg-GCUGGUaccgggcuccGGUAUCGAGGAggCUCa -3' miRNA: 3'- -UGGagCGACCG----------CCGUGGUUCCUa-GAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 22896 | 1.09 | 0.000378 |
Target: 5'- gACCUCGCUGGCGGCACCAAGGAUCUCu -3' miRNA: 3'- -UGGAGCGACCGCCGUGGUUCCUAGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 8600 | 0.72 | 0.202351 |
Target: 5'- uCCUCGCUGaCGGUuuCCGcgucGGGGUCUCa -3' miRNA: 3'- uGGAGCGACcGCCGu-GGU----UCCUAGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 25057 | 0.7 | 0.262728 |
Target: 5'- gGCCgCGCUGGUaGCACCugugccgccguuGGGGAUCUg -3' miRNA: 3'- -UGGaGCGACCGcCGUGG------------UUCCUAGAg -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 46812 | 0.69 | 0.328888 |
Target: 5'- gACCUCGacCUGGCcgucacccGaGUACuCAAGGAUCUCc -3' miRNA: 3'- -UGGAGC--GACCG--------C-CGUG-GUUCCUAGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 39371 | 0.69 | 0.336142 |
Target: 5'- gUCUCGUUGGgGGUguagagcguggcaGCCu-GGAUCUCg -3' miRNA: 3'- uGGAGCGACCgCCG-------------UGGuuCCUAGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 23596 | 0.69 | 0.340222 |
Target: 5'- aGCCaCGCUGGCGGCucaggccggugcgacGaCCAAGGAgCUg -3' miRNA: 3'- -UGGaGCGACCGCCG---------------U-GGUUCCUaGAg -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 15973 | 0.68 | 0.388338 |
Target: 5'- aACCUgGgUGGCGGCAgCAAGccGAaCUCc -3' miRNA: 3'- -UGGAgCgACCGCCGUgGUUC--CUaGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 17022 | 0.67 | 0.425336 |
Target: 5'- aACCUCGCcaacGGCcGCuACCAAGGugCUCa -3' miRNA: 3'- -UGGAGCGa---CCGcCG-UGGUUCCuaGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 28330 | 0.66 | 0.464353 |
Target: 5'- aGCgUgaGCUGGUGGUucUCAAGGAUCUg -3' miRNA: 3'- -UGgAg-CGACCGCCGu-GGUUCCUAGAg -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 31306 | 0.66 | 0.464353 |
Target: 5'- cGCCUgcaGCgcaGUGGUaagcgcaguGCCGAGGGUCUCa -3' miRNA: 3'- -UGGAg--CGac-CGCCG---------UGGUUCCUAGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 20895 | 0.66 | 0.494805 |
Target: 5'- cGCCgaggCGCUGcUGGagaACCAAGGG-CUCa -3' miRNA: 3'- -UGGa---GCGACcGCCg--UGGUUCCUaGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 18558 | 0.74 | 0.138271 |
Target: 5'- uCCUCGCUGGUaucaGCACCAAGGAa--- -3' miRNA: 3'- uGGAGCGACCGc---CGUGGUUCCUagag -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 35737 | 0.7 | 0.262728 |
Target: 5'- gGCCUcCGCUGGcCGuaGCCAGGGGUg-- -3' miRNA: 3'- -UGGA-GCGACC-GCcgUGGUUCCUAgag -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 34805 | 0.69 | 0.320964 |
Target: 5'- cGCCuUCGCgGGCa-CACCGgcucAGGAUCUCg -3' miRNA: 3'- -UGG-AGCGaCCGccGUGGU----UCCUAGAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 26937 | 0.69 | 0.328887 |
Target: 5'- cACCgCGCUGGCGaGCugucagcgGCCAAcGGAguggCUCa -3' miRNA: 3'- -UGGaGCGACCGC-CG--------UGGUU-CCUa---GAG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 24723 | 0.68 | 0.353518 |
Target: 5'- cAUCggCGCUGGUGGUACCGcAGGGcCUg -3' miRNA: 3'- -UGGa-GCGACCGCCGUGGU-UCCUaGAg -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 44402 | 0.67 | 0.444605 |
Target: 5'- aGCUcgaUCGCUGGC-GUACCGuccGGGGUCa- -3' miRNA: 3'- -UGG---AGCGACCGcCGUGGU---UCCUAGag -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 30912 | 0.66 | 0.464353 |
Target: 5'- uCCUCGaugGGCaGCACCcAGGAUUcgUCu -3' miRNA: 3'- uGGAGCga-CCGcCGUGGuUCCUAG--AG- -5' |
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7200 | 5' | -58.5 | NC_001900.1 | + | 1241 | 0.66 | 0.48455 |
Target: 5'- -aCUCGgUGGCGGCGgCAGcGGAgCUg -3' miRNA: 3'- ugGAGCgACCGCCGUgGUU-CCUaGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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