Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7201 | 3' | -55.2 | NC_001900.1 | + | 13778 | 0.7 | 0.42709 |
Target: 5'- -cUGUggGCGUCGgGCGUCAagaacGCCCa- -3' miRNA: 3'- ucACGuuCGCAGCaUGCAGU-----CGGGcu -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 17423 | 0.66 | 0.660075 |
Target: 5'- cGGUG-AAGCGuUCGUcaaaggcagcuugGCuccgGUCGGCCCGAa -3' miRNA: 3'- -UCACgUUCGC-AGCA-------------UG----CAGUCGGGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 21923 | 0.67 | 0.650099 |
Target: 5'- -uUGCAGGCGUCGggGCGaUCAcGCauCCGGc -3' miRNA: 3'- ucACGUUCGCAGCa-UGC-AGU-CG--GGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 22684 | 1.09 | 0.000897 |
Target: 5'- gAGUGCAAGCGUCGUACGUCAGCCCGAu -3' miRNA: 3'- -UCACGUUCGCAGCAUGCAGUCGGGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 23892 | 0.66 | 0.672238 |
Target: 5'- --gGCAGGCucgccUCGUACc-CGGCCCGAg -3' miRNA: 3'- ucaCGUUCGc----AGCAUGcaGUCGGGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 28241 | 0.67 | 0.627892 |
Target: 5'- cGUcGguAGCGUCGUGCaGUCgacggaucaGGUCCGGg -3' miRNA: 3'- uCA-CguUCGCAGCAUG-CAG---------UCGGGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 37368 | 0.67 | 0.650099 |
Target: 5'- cGGUGUGAGCGUCGUuCG-CcGCCuUGAc -3' miRNA: 3'- -UCACGUUCGCAGCAuGCaGuCGG-GCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 38992 | 0.66 | 0.672238 |
Target: 5'- gAGUGCGuuGGUGaaGUugGcCAGCUCGGc -3' miRNA: 3'- -UCACGU--UCGCagCAugCaGUCGGGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 39897 | 0.67 | 0.627892 |
Target: 5'- cGUGCAgcagcGGUGUCGUGuCGgcuucaUCAGCgCGAa -3' miRNA: 3'- uCACGU-----UCGCAGCAU-GC------AGUCGgGCU- -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 40804 | 0.69 | 0.491231 |
Target: 5'- gGGUGUcggccggcgcuucgaGAGCGUCGUACaUCAuGCCUGu -3' miRNA: 3'- -UCACG---------------UUCGCAGCAUGcAGU-CGGGCu -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 44040 | 0.72 | 0.354351 |
Target: 5'- cGUGCAAGCGgUGUGCGaguacCGGCCCc- -3' miRNA: 3'- uCACGUUCGCaGCAUGCa----GUCGGGcu -5' |
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7201 | 3' | -55.2 | NC_001900.1 | + | 49007 | 0.66 | 0.661183 |
Target: 5'- uGG-GCgAGGCGcUCuaGCGUCGGCCCGu -3' miRNA: 3'- -UCaCG-UUCGC-AGcaUGCAGUCGGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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