Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7202 | 5' | -54.9 | NC_001900.1 | + | 28018 | 0.66 | 0.675124 |
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Target: 5'- gUUCgGGCCGgUGUaACCg--GGUGCGg -3' miRNA: 3'- -AAGgCCGGCaACGcUGGaauUCACGC- -5' |
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| 7202 | 5' | -54.9 | NC_001900.1 | + | 40321 | 0.67 | 0.607832 |
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Target: 5'- cUgCGGCCGUgguUGGCCgcgucGAGUGCGa -3' miRNA: 3'- aAgGCCGGCAac-GCUGGaa---UUCACGC- -5' |
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| 7202 | 5' | -54.9 | NC_001900.1 | + | 35107 | 0.68 | 0.57434 |
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Target: 5'- -aCCGGaCUGguugGCGACCUggaagcGUGCGa -3' miRNA: 3'- aaGGCC-GGCaa--CGCUGGAauu---CACGC- -5' |
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| 7202 | 5' | -54.9 | NC_001900.1 | + | 18665 | 0.69 | 0.508901 |
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Target: 5'- -gCCGGCCG-UGCGGCgCUgcAG-GCGc -3' miRNA: 3'- aaGGCCGGCaACGCUG-GAauUCaCGC- -5' |
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| 7202 | 5' | -54.9 | NC_001900.1 | + | 41933 | 0.77 | 0.164664 |
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Target: 5'- aUCCGGCCGaugGCGACCUcguaUucGUGCa -3' miRNA: 3'- aAGGCCGGCaa-CGCUGGA----AuuCACGc -5' |
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| 7202 | 5' | -54.9 | NC_001900.1 | + | 22189 | 1.07 | 0.001165 |
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Target: 5'- gUUCCGGCCGUUGCGACCUUAAGUGCGa -3' miRNA: 3'- -AAGGCCGGCAACGCUGGAAUUCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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