Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7205 | 5' | -53 | NC_001900.1 | + | 48987 | 0.67 | 0.731731 |
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Target: 5'- cGAGGCAcGCGUGCcgugaGCgUUUGCUGUGc -3' miRNA: 3'- aCUCCGUuUGCGUGa----CG-AGAUGACGC- -5' |
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| 7205 | 5' | -53 | NC_001900.1 | + | 35757 | 0.68 | 0.675845 |
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Target: 5'- aGGGGUGaacacgccgccGACGUACUGCUCcaggucgucCUGCGa -3' miRNA: 3'- aCUCCGU-----------UUGCGUGACGAGau-------GACGC- -5' |
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| 7205 | 5' | -53 | NC_001900.1 | + | 32884 | 0.69 | 0.596037 |
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Target: 5'- cUGGGGUAcugcgcGGgGCACUGCUCgu-UGCGa -3' miRNA: 3'- -ACUCCGU------UUgCGUGACGAGaugACGC- -5' |
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| 7205 | 5' | -53 | NC_001900.1 | + | 24812 | 0.69 | 0.573374 |
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Target: 5'- -cGGGCGGugGCAC-GCUCaucGCUGCc -3' miRNA: 3'- acUCCGUUugCGUGaCGAGa--UGACGc -5' |
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| 7205 | 5' | -53 | NC_001900.1 | + | 16136 | 0.72 | 0.414117 |
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Target: 5'- cGGGGaacaccuACGCGCUGUUCUcCUGCa -3' miRNA: 3'- aCUCCguu----UGCGUGACGAGAuGACGc -5' |
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| 7205 | 5' | -53 | NC_001900.1 | + | 13444 | 0.77 | 0.218055 |
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Target: 5'- cGAGGCcGACGCgguGCUGCgUCUGgUGCGu -3' miRNA: 3'- aCUCCGuUUGCG---UGACG-AGAUgACGC- -5' |
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| 7205 | 5' | -53 | NC_001900.1 | + | 21368 | 1.1 | 0.001106 |
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Target: 5'- cUGAGGCAAACGCACUGCUCUACUGCGa -3' miRNA: 3'- -ACUCCGUUUGCGUGACGAGAUGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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