Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7209 | 5' | -53.2 | NC_001900.1 | + | 19446 | 0.66 | 0.799534 |
Target: 5'- gUGAUGUcGuGCaUCUCGUACGACC-Cg -3' miRNA: 3'- -ACUACGuCuUGcGGAGCAUGCUGGaG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 7671 | 0.66 | 0.789547 |
Target: 5'- gGgcGCAGGGCGUCacCGUACguGACCUa -3' miRNA: 3'- aCuaCGUCUUGCGGa-GCAUG--CUGGAg -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 31370 | 0.66 | 0.769068 |
Target: 5'- aGAgccUGCAGAGCGCCcgucagcaUCGUGCcgagggugucGGCCa- -3' miRNA: 3'- aCU---ACGUCUUGCGG--------AGCAUG----------CUGGag -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 42244 | 0.66 | 0.769068 |
Target: 5'- ---cGUAGAGCGCgUCGUACG-CUg- -3' miRNA: 3'- acuaCGUCUUGCGgAGCAUGCuGGag -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 7576 | 0.66 | 0.758599 |
Target: 5'- cGAagGCGcGAACGUCUCcgACGACUUCu -3' miRNA: 3'- aCUa-CGU-CUUGCGGAGcaUGCUGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 27175 | 0.67 | 0.747993 |
Target: 5'- aGAuUGCGGAGCGCCcagaCG-ACGugCUg -3' miRNA: 3'- aCU-ACGUCUUGCGGa---GCaUGCugGAg -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 8256 | 0.67 | 0.747993 |
Target: 5'- cGGUGUGGGACGCCcUGgcugACGAgCUg -3' miRNA: 3'- aCUACGUCUUGCGGaGCa---UGCUgGAg -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 44100 | 0.67 | 0.730772 |
Target: 5'- cGAUGCcuuccucgacccuguGGAACuCCUCGgugAUGGCUUCa -3' miRNA: 3'- aCUACG---------------UCUUGcGGAGCa--UGCUGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 21268 | 0.67 | 0.726423 |
Target: 5'- gGAgcUGCAGGAUGCa--GUACGugcCCUCg -3' miRNA: 3'- aCU--ACGUCUUGCGgagCAUGCu--GGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 23888 | 0.67 | 0.726423 |
Target: 5'- cGucgGCAGGcuCGCCUCGUAccCGGCC-Cg -3' miRNA: 3'- aCua-CGUCUu-GCGGAGCAU--GCUGGaG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 3518 | 0.67 | 0.704457 |
Target: 5'- cGGUGacaucCAGGACGCCaUCGacGCGGCgCUCg -3' miRNA: 3'- aCUAC-----GUCUUGCGG-AGCa-UGCUG-GAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 33167 | 0.68 | 0.691128 |
Target: 5'- -aAUGCGGAGCGaguaCUCGUccaccagcuccuCGGCCUCc -3' miRNA: 3'- acUACGUCUUGCg---GAGCAu-----------GCUGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 31070 | 0.68 | 0.670979 |
Target: 5'- --uUGguGAACGCCUgG-ACGAUCUg -3' miRNA: 3'- acuACguCUUGCGGAgCaUGCUGGAg -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 41083 | 0.68 | 0.659727 |
Target: 5'- cGAUGCugguGAGgagguugcUGCCUCGaUGCGGCCa- -3' miRNA: 3'- aCUACGu---CUU--------GCGGAGC-AUGCUGGag -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 28208 | 0.69 | 0.614559 |
Target: 5'- cGAUGUcGuugGCCUCGUccuugcggACGGCCUCg -3' miRNA: 3'- aCUACGuCuugCGGAGCA--------UGCUGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 32344 | 0.69 | 0.580813 |
Target: 5'- aGAUGU-GGACGagguaCUCGUACG-CCUCc -3' miRNA: 3'- aCUACGuCUUGCg----GAGCAUGCuGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 6289 | 0.7 | 0.569642 |
Target: 5'- cGG-GCuGGACGCCUgGcACGACCUg -3' miRNA: 3'- aCUaCGuCUUGCGGAgCaUGCUGGAg -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 48163 | 0.7 | 0.558524 |
Target: 5'- ---cGCGGAaaaGCGCCUgGU-CGACCUUc -3' miRNA: 3'- acuaCGUCU---UGCGGAgCAuGCUGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 35916 | 0.7 | 0.558524 |
Target: 5'- aGgcGCuGGGCGUCUCGUcggaGACCUCc -3' miRNA: 3'- aCuaCGuCUUGCGGAGCAug--CUGGAG- -5' |
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7209 | 5' | -53.2 | NC_001900.1 | + | 31085 | 0.71 | 0.493389 |
Target: 5'- uUGAUGguGAcgAgGUCUCcUugGACCUCa -3' miRNA: 3'- -ACUACguCU--UgCGGAGcAugCUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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