Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7210 | 5' | -56.1 | NC_001900.1 | + | 25725 | 0.68 | 0.530964 |
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Target: 5'- aUCCGUaGCCGCCg---CCGGUauagaacUCGGc -3' miRNA: 3'- -AGGCAaCGGCGGaucaGGUCA-------AGCCa -5' |
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| 7210 | 5' | -56.1 | NC_001900.1 | + | 24149 | 0.68 | 0.510843 |
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Target: 5'- gCCuagcGCCGCCgucguaGGUCCGGUcUCGGUa -3' miRNA: 3'- aGGcaa-CGGCGGa-----UCAGGUCA-AGCCA- -5' |
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| 7210 | 5' | -56.1 | NC_001900.1 | + | 43624 | 0.68 | 0.490014 |
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Target: 5'- cUCCGgggUGuuGCCgAGgUCGGUUCGGg -3' miRNA: 3'- -AGGCa--ACggCGGaUCaGGUCAAGCCa -5' |
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| 7210 | 5' | -56.1 | NC_001900.1 | + | 39232 | 0.69 | 0.437848 |
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Target: 5'- cUCCGgggugaucucagaggUGCCguacagguccgagaGCCgGGUCCGGUUCGGa -3' miRNA: 3'- -AGGCa--------------ACGG--------------CGGaUCAGGUCAAGCCa -5' |
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| 7210 | 5' | -56.1 | NC_001900.1 | + | 43095 | 0.7 | 0.420543 |
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Target: 5'- gCCGcUUGCgGCCUGG-CCGGUUCa-- -3' miRNA: 3'- aGGC-AACGgCGGAUCaGGUCAAGcca -5' |
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| 7210 | 5' | -56.1 | NC_001900.1 | + | 18116 | 1.08 | 0.000804 |
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Target: 5'- uUCCGUUGCCGCCUAGUCCAGUUCGGUa -3' miRNA: 3'- -AGGCAACGGCGGAUCAGGUCAAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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