Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7213 | 3' | -55.2 | NC_001900.1 | + | 323 | 0.66 | 0.671298 |
Target: 5'- --gGGCcGGGGAAUUGaucCGGCAaccGCc -3' miRNA: 3'- ugaCCGuCCCCUUAACgaaGCCGU---CG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 14164 | 0.66 | 0.660095 |
Target: 5'- cGCguccgGGCGGcGGGucgcaaGCUgaagacguUCGGCGGCa -3' miRNA: 3'- -UGa----CCGUC-CCCuuaa--CGA--------AGCCGUCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 23279 | 0.67 | 0.592689 |
Target: 5'- cGCUGGC-GGGGA--UGaCUUCGaGCgAGUc -3' miRNA: 3'- -UGACCGuCCCCUuaAC-GAAGC-CG-UCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 24624 | 0.69 | 0.451098 |
Target: 5'- cACUGcGUGGGGccaaggcgGCUUCGGCGGUu -3' miRNA: 3'- -UGAC-CGUCCCcuuaa---CGAAGCCGUCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 1262 | 0.71 | 0.36764 |
Target: 5'- aGCUGGCGGcGGGuucggUGgUUCgaccGGCAGCc -3' miRNA: 3'- -UGACCGUC-CCCuua--ACgAAG----CCGUCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 20902 | 0.71 | 0.35885 |
Target: 5'- uCUGGCuGGGGcaccGAgaccGcCUUCGGCGGCu -3' miRNA: 3'- uGACCGuCCCC----UUaa--C-GAAGCCGUCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 33339 | 0.71 | 0.350208 |
Target: 5'- uGCUgGGCAGGGGGAUcccaggGCgacucagUCGGCGucuGCu -3' miRNA: 3'- -UGA-CCGUCCCCUUAa-----CGa------AGCCGU---CG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 45885 | 0.75 | 0.202922 |
Target: 5'- --cGGCGGGGGA---GCagUCGGUAGCg -3' miRNA: 3'- ugaCCGUCCCCUuaaCGa-AGCCGUCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 38304 | 0.83 | 0.054031 |
Target: 5'- uCUGGCAuGGGGAugauucgUGCgUUCGGCAGCg -3' miRNA: 3'- uGACCGU-CCCCUua-----ACG-AAGCCGUCG- -5' |
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7213 | 3' | -55.2 | NC_001900.1 | + | 16905 | 1.11 | 0.000516 |
Target: 5'- aACUGGCAGGGGAAUUGCUUCGGCAGCu -3' miRNA: 3'- -UGACCGUCCCCUUAACGAAGCCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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