Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7213 | 5' | -54.4 | NC_001900.1 | + | 24227 | 0.66 | 0.730125 |
Target: 5'- aCGCUCaCCGACAUC--CCAGuGGCAg- -3' miRNA: 3'- aGCGAG-GGUUGUGGauGGUC-CUGUag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 10776 | 0.66 | 0.730125 |
Target: 5'- -aGCUgCCGAC-CUggGCCGGGACuUCu -3' miRNA: 3'- agCGAgGGUUGuGGa-UGGUCCUGuAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 18068 | 0.66 | 0.718191 |
Target: 5'- cUCGCcgUCGACACCgaaACCAccgggcuGGACAUCu -3' miRNA: 3'- -AGCGagGGUUGUGGa--UGGU-------CCUGUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 794 | 0.66 | 0.708346 |
Target: 5'- gUGCUCCCAAcCGCgUGCU-GGGCGa- -3' miRNA: 3'- aGCGAGGGUU-GUGgAUGGuCCUGUag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 39127 | 0.66 | 0.708346 |
Target: 5'- gCGCUCCCcgAugACCUggacgGCCGGGGg--- -3' miRNA: 3'- aGCGAGGG--UugUGGA-----UGGUCCUguag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 19076 | 0.66 | 0.708346 |
Target: 5'- gCGCUUCaacuuGACgACCggugACCAGGGCGUg -3' miRNA: 3'- aGCGAGGg----UUG-UGGa---UGGUCCUGUAg -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 30928 | 0.66 | 0.686256 |
Target: 5'- cUGCg-CCGACGagUCUGCCAGGACGc- -3' miRNA: 3'- aGCGagGGUUGU--GGAUGGUCCUGUag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 34811 | 0.67 | 0.66395 |
Target: 5'- cUCGCUCCUccuuGACcCgCUugC-GGACAUCg -3' miRNA: 3'- -AGCGAGGG----UUGuG-GAugGuCCUGUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 14887 | 0.67 | 0.641519 |
Target: 5'- -aGCUCCC----UCUACCAGGAC-UCc -3' miRNA: 3'- agCGAGGGuuguGGAUGGUCCUGuAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 29510 | 0.68 | 0.585467 |
Target: 5'- -gGCgaaUCAGCGCCUugCAGGucACAUCc -3' miRNA: 3'- agCGag-GGUUGUGGAugGUCC--UGUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 14431 | 0.69 | 0.541291 |
Target: 5'- aCGUUCaCCAACAUCaAgCAGGACAg- -3' miRNA: 3'- aGCGAG-GGUUGUGGaUgGUCCUGUag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 2413 | 0.69 | 0.519612 |
Target: 5'- -gGCUCCCGGuccuuuCugCUGCCGGGugGc- -3' miRNA: 3'- agCGAGGGUU------GugGAUGGUCCugUag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 30282 | 0.69 | 0.508901 |
Target: 5'- -aGCUCCUcgAACGCCU-CCGcGACAUCc -3' miRNA: 3'- agCGAGGG--UUGUGGAuGGUcCUGUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 21175 | 0.7 | 0.498283 |
Target: 5'- cCGCUCCgGGCAUCaACgAGGGgAUCu -3' miRNA: 3'- aGCGAGGgUUGUGGaUGgUCCUgUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 429 | 0.7 | 0.498283 |
Target: 5'- -aGCUCaCAGCACCcaUACCGGGcACAUa -3' miRNA: 3'- agCGAGgGUUGUGG--AUGGUCC-UGUAg -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 21392 | 0.7 | 0.446806 |
Target: 5'- cUCGCUUCCcAUugCgggGCUugAGGACAUCg -3' miRNA: 3'- -AGCGAGGGuUGugGa--UGG--UCCUGUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 35891 | 0.71 | 0.417376 |
Target: 5'- aCGCUUCCAAcCGCCaGCCGcgaucaggcgcuGGGCGUCu -3' miRNA: 3'- aGCGAGGGUU-GUGGaUGGU------------CCUGUAG- -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 15420 | 0.71 | 0.407831 |
Target: 5'- gUCGaC-CCgGACGCCUACgAGGGCGUg -3' miRNA: 3'- -AGC-GaGGgUUGUGGAUGgUCCUGUAg -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 41206 | 0.73 | 0.30455 |
Target: 5'- gCGCgCCCAGCACCUGCUGGuGCAg- -3' miRNA: 3'- aGCGaGGGUUGUGGAUGGUCcUGUag -5' |
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7213 | 5' | -54.4 | NC_001900.1 | + | 27148 | 0.74 | 0.274889 |
Target: 5'- aUCGC-CCCGACGCCUGCaaucaaCGGGAgAUUg -3' miRNA: 3'- -AGCGaGGGUUGUGGAUG------GUCCUgUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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