Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7214 | 3' | -52 | NC_001900.1 | + | 10489 | 0.7 | 0.648866 |
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Target: 5'- gCCGAaguACAUGACCA---UGCCGggGGa -3' miRNA: 3'- -GGCU---UGUACUGGUugaGCGGCuaCCa -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 14588 | 0.7 | 0.648866 |
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Target: 5'- uCCGAGaa-GACCcucGACUCGCCGG-GGUc -3' miRNA: 3'- -GGCUUguaCUGG---UUGAGCGGCUaCCA- -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 19473 | 0.71 | 0.603893 |
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Target: 5'- cCCGAug--GGCCGGCggcUGCCGGUGGa -3' miRNA: 3'- -GGCUuguaCUGGUUGa--GCGGCUACCa -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 8018 | 0.71 | 0.570393 |
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Target: 5'- -aGAACGUcGACCGACUgGCCaagaGGUGGa -3' miRNA: 3'- ggCUUGUA-CUGGUUGAgCGG----CUACCa -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 1786 | 0.72 | 0.537364 |
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Target: 5'- aCCGggUcacuGUGACCAucggcgACUCGCUGAUGu- -3' miRNA: 3'- -GGCuuG----UACUGGU------UGAGCGGCUACca -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 45143 | 0.72 | 0.537364 |
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Target: 5'- aCCG-GCGUcucGGCCAGCUCGCCGAc--- -3' miRNA: 3'- -GGCuUGUA---CUGGUUGAGCGGCUacca -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 20878 | 0.76 | 0.341715 |
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Target: 5'- aUCGcACAaGGCCAGCUCGCCGA-GGc -3' miRNA: 3'- -GGCuUGUaCUGGUUGAGCGGCUaCCa -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 2931 | 0.77 | 0.293915 |
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Target: 5'- cCUGGuCAUcGACCAGCUCGgCGAUGGa -3' miRNA: 3'- -GGCUuGUA-CUGGUUGAGCgGCUACCa -5' |
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| 7214 | 3' | -52 | NC_001900.1 | + | 16801 | 1.09 | 0.002104 |
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Target: 5'- gCCGAACAUGACCAACUCGCCGAUGGUc -3' miRNA: 3'- -GGCUUGUACUGGUUGAGCGGCUACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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