Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7216 | 3' | -55.7 | NC_001900.1 | + | 25913 | 0.66 | 0.677894 |
Target: 5'- uCAACGACGGuCUGGUCCUCgcccgGGCUg -3' miRNA: 3'- -GUUGCUGCU-GACCGGGGGagug-UUGA- -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 12611 | 0.66 | 0.666881 |
Target: 5'- gAACGACGGCaagaagUGGacccacaCCCUUCugGACg -3' miRNA: 3'- gUUGCUGCUG------ACCg------GGGGAGugUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 27965 | 0.66 | 0.652514 |
Target: 5'- --cCGACGACuugaucccggucagUGGCCCCuCUucgaaCACGACg -3' miRNA: 3'- guuGCUGCUG--------------ACCGGGG-GA-----GUGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 27866 | 0.66 | 0.622601 |
Target: 5'- uGACGACGACUGcaccaaccuuGCuCCUUUCACAGu- -3' miRNA: 3'- gUUGCUGCUGAC----------CG-GGGGAGUGUUga -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 8423 | 0.67 | 0.611527 |
Target: 5'- -cACGGCaagaaGGCUGGCgUCCUcCGCAACa -3' miRNA: 3'- guUGCUG-----CUGACCGgGGGA-GUGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 48211 | 0.67 | 0.600472 |
Target: 5'- uGAUcGCGACcgUGGCCCCCUC-CGGg- -3' miRNA: 3'- gUUGcUGCUG--ACCGGGGGAGuGUUga -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 2720 | 0.67 | 0.599368 |
Target: 5'- -cGCGACGACUGGCgcggcaaCCCgUCcgcgugggACGACg -3' miRNA: 3'- guUGCUGCUGACCG-------GGGgAG--------UGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 32018 | 0.67 | 0.567505 |
Target: 5'- aGACGGCGGucUUGGCCgCUUC-CAGCg -3' miRNA: 3'- gUUGCUGCU--GACCGGgGGAGuGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 5971 | 0.67 | 0.567505 |
Target: 5'- aCGGCGACGGCauggGGCCgCC-CGCuACg -3' miRNA: 3'- -GUUGCUGCUGa---CCGGgGGaGUGuUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 7296 | 0.68 | 0.535014 |
Target: 5'- gUAugGGCGACaagUGGCCgCaCCUCuACAACc -3' miRNA: 3'- -GUugCUGCUG---ACCGG-G-GGAG-UGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 5050 | 0.68 | 0.503203 |
Target: 5'- uGAUGACGAUggacauccccggUGGCCCggugaaggUCUCGCAACg -3' miRNA: 3'- gUUGCUGCUG------------ACCGGG--------GGAGUGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 44681 | 0.69 | 0.49278 |
Target: 5'- aCAGCGugGAacgcGGUCCCCU-GCGGCa -3' miRNA: 3'- -GUUGCugCUga--CCGGGGGAgUGUUGa -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 43308 | 0.69 | 0.462136 |
Target: 5'- ---aGACGuugcucacGCUGGCUCCCUCACGu-- -3' miRNA: 3'- guugCUGC--------UGACCGGGGGAGUGUuga -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 157 | 0.7 | 0.432524 |
Target: 5'- -uGCGACGAUgcgaaugccGGCCUCagCUCACAGCUa -3' miRNA: 3'- guUGCUGCUGa--------CCGGGG--GAGUGUUGA- -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 29246 | 0.7 | 0.422901 |
Target: 5'- cCGACGGCGAC-GaGCCaCCCUCuuGGCUu -3' miRNA: 3'- -GUUGCUGCUGaC-CGG-GGGAGugUUGA- -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 20147 | 0.7 | 0.394823 |
Target: 5'- gCGACGGCGACUuccgucugcgcGGCCUCgUCGCGGgUg -3' miRNA: 3'- -GUUGCUGCUGA-----------CCGGGGgAGUGUUgA- -5' |
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7216 | 3' | -55.7 | NC_001900.1 | + | 16249 | 1.07 | 0.001142 |
Target: 5'- gCAACGACGACUGGCCCCCUCACAACUc -3' miRNA: 3'- -GUUGCUGCUGACCGGGGGAGUGUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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