Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7219 | 3' | -57.4 | NC_001900.1 | + | 444 | 0.67 | 0.464353 |
Target: 5'- uACC--GGGCACAUaacgagCAGCCUaugcaCGCGGCu -3' miRNA: 3'- -UGGauCCCGUGUA------GUUGGA-----GCGCCGc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 2950 | 0.7 | 0.353518 |
Target: 5'- gGCgaUGGaGGuCACAUCuACCuUCGCGGCGa -3' miRNA: 3'- -UGg-AUC-CC-GUGUAGuUGG-AGCGCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 5911 | 0.77 | 0.11373 |
Target: 5'- uCCUGGGGgACAUCAGCuCagGCGGCa -3' miRNA: 3'- uGGAUCCCgUGUAGUUG-GagCGCCGc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 6408 | 0.67 | 0.494805 |
Target: 5'- cACCaGGGGCgucgaGCAg-GugCUCGCGGCc -3' miRNA: 3'- -UGGaUCCCG-----UGUagUugGAGCGCCGc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 8300 | 0.72 | 0.249579 |
Target: 5'- gGCCUGGuGGUgguucACggCGGCgCUCGCGGCGc -3' miRNA: 3'- -UGGAUC-CCG-----UGuaGUUG-GAGCGCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 14710 | 1.08 | 0.000629 |
Target: 5'- aACCUAGGGCACAUCAACCUCGCGGCGu -3' miRNA: 3'- -UGGAUCCCGUGUAGUUGGAGCGCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 23645 | 0.7 | 0.353518 |
Target: 5'- cGCCUcGGGCACA-CGACUccgCGCauGGCGa -3' miRNA: 3'- -UGGAuCCCGUGUaGUUGGa--GCG--CCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 27413 | 0.76 | 0.127208 |
Target: 5'- uACCgaUGGGGCGCAUCAgcGCCUCGaCGGa- -3' miRNA: 3'- -UGG--AUCCCGUGUAGU--UGGAGC-GCCgc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 27463 | 0.69 | 0.362013 |
Target: 5'- aGCCUGGGGCcgagccGCA-CGGCCUUGagccggccguCGGCGa -3' miRNA: 3'- -UGGAUCCCG------UGUaGUUGGAGC----------GCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 31398 | 0.66 | 0.526134 |
Target: 5'- uGCCgAGGGU--GUCGGCCagCGUGGUGa -3' miRNA: 3'- -UGGaUCCCGugUAGUUGGa-GCGCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 31738 | 0.68 | 0.444604 |
Target: 5'- cACCUGGcGGUugaccGCAgugacCAACCgaccguUCGCGGCGg -3' miRNA: 3'- -UGGAUC-CCG-----UGUa----GUUGG------AGCGCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 33475 | 0.66 | 0.547429 |
Target: 5'- cCCUccGGGGUAgA-CAGCCUgGUGGCu -3' miRNA: 3'- uGGA--UCCCGUgUaGUUGGAgCGCCGc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 38594 | 0.69 | 0.370649 |
Target: 5'- gGCCUcuuccucgucccAGcGGCGCAUCu-CCUCGCgcucGGCGa -3' miRNA: 3'- -UGGA------------UC-CCGUGUAGuuGGAGCG----CCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 38700 | 0.74 | 0.172203 |
Target: 5'- aGCUUcGuGGCgGCGUCAGCCuUCGCGGCGu -3' miRNA: 3'- -UGGAuC-CCG-UGUAGUUGG-AGCGCCGC- -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 40711 | 0.68 | 0.454421 |
Target: 5'- cGCCUcGGGCG-AUCAAcagccCCUCGCGGa- -3' miRNA: 3'- -UGGAuCCCGUgUAGUU-----GGAGCGCCgc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 42966 | 0.72 | 0.262057 |
Target: 5'- cGCCUccgucgaGGGGgACGcgaAGCCUCGCGGCc -3' miRNA: 3'- -UGGA-------UCCCgUGUag-UUGGAGCGCCGc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 46623 | 0.67 | 0.505158 |
Target: 5'- aGCC-GGGGC-CGUCGACCUCacacagccaccCGGCa -3' miRNA: 3'- -UGGaUCCCGuGUAGUUGGAGc----------GCCGc -5' |
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7219 | 3' | -57.4 | NC_001900.1 | + | 48562 | 0.67 | 0.48864 |
Target: 5'- cACgUAGGGCACGUguggcaggccagacgCAACCcacuaGCGGCc -3' miRNA: 3'- -UGgAUCCCGUGUA---------------GUUGGag---CGCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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