Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7221 | 3' | -55.2 | NC_001900.1 | + | 18104 | 0.66 | 0.6854 |
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Target: 5'- ----uUCCUGaucgguggcaucuCCCgGCUGGUUGGCGUCc -3' miRNA: 3'- guucuAGGAC-------------GGG-CGACCAGCUGUAG- -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 8647 | 0.66 | 0.675423 |
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Target: 5'- cCGGGucUCCgacgGCCUGCUGuucgucaUCGACAUCu -3' miRNA: 3'- -GUUCu-AGGa---CGGGCGACc------AGCUGUAG- -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 1372 | 0.66 | 0.672089 |
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Target: 5'- cCGAGAUCgCUGCagagcgugaggcugCC-CUGGUCGAagaCAUCa -3' miRNA: 3'- -GUUCUAG-GACG--------------GGcGACCAGCU---GUAG- -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 31782 | 0.67 | 0.619595 |
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Target: 5'- uCAGGAacgCCUGCgUGCcGGUCGAgAUg -3' miRNA: 3'- -GUUCUa--GGACGgGCGaCCAGCUgUAg -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 30302 | 0.68 | 0.564035 |
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Target: 5'- -cGGGUCaucGCCCGCUGcGcCGACGUa -3' miRNA: 3'- guUCUAGga-CGGGCGAC-CaGCUGUAg -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 38532 | 0.71 | 0.390475 |
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Target: 5'- gGGGAgCCUGggaCCGUcgGGUCGGCGUCg -3' miRNA: 3'- gUUCUaGGACg--GGCGa-CCAGCUGUAG- -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 17791 | 0.71 | 0.390475 |
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Target: 5'- --cGAUgCUGCCCacGCUGGUgGACAg- -3' miRNA: 3'- guuCUAgGACGGG--CGACCAgCUGUag -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 30052 | 0.71 | 0.390475 |
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Target: 5'- gCGAGGUCCgcGCCUGCagcgGGUUGACGa- -3' miRNA: 3'- -GUUCUAGGa-CGGGCGa---CCAGCUGUag -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 21075 | 0.72 | 0.321702 |
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Target: 5'- --uGAUCUucgUGCCCGCcGGgggCGGCGUCa -3' miRNA: 3'- guuCUAGG---ACGGGCGaCCa--GCUGUAG- -5' |
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| 7221 | 3' | -55.2 | NC_001900.1 | + | 13752 | 1.09 | 0.000834 |
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Target: 5'- uCAAGAUCCUGCCCGCUGGUCGACAUCu -3' miRNA: 3'- -GUUCUAGGACGGGCGACCAGCUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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