Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7221 | 5' | -57.9 | NC_001900.1 | + | 48393 | 0.66 | 0.539509 |
Target: 5'- uCGGGCGUC-AGAGCaGCuCCAacCGCUUGc -3' miRNA: 3'- -GCCCGCAGuUCUUG-CG-GGUc-GUGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 16887 | 0.66 | 0.539509 |
Target: 5'- -cGGCGUCGGGAucguCGCCaucgccGCGCCg- -3' miRNA: 3'- gcCCGCAGUUCUu---GCGGgu----CGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 44280 | 0.66 | 0.52893 |
Target: 5'- uCGGGgGUUGAguguGAACGCCCcgccguagccGUACCUGu -3' miRNA: 3'- -GCCCgCAGUU----CUUGCGGGu---------CGUGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 46480 | 0.66 | 0.525772 |
Target: 5'- cCGGGCcuugaguucccgccGUCGGGcuACGCCCucAGCGgCUGa -3' miRNA: 3'- -GCCCG--------------CAGUUCu-UGCGGG--UCGUgGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 35283 | 0.66 | 0.51843 |
Target: 5'- uGGGCGUUcuuGACGCCCGaCGCCc- -3' miRNA: 3'- gCCCGCAGuucUUGCGGGUcGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 31773 | 0.66 | 0.508012 |
Target: 5'- uGGacaaCGUCAGGAACGCCUGcGUGCCg- -3' miRNA: 3'- gCCc---GCAGUUCUUGCGGGU-CGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 37088 | 0.67 | 0.497684 |
Target: 5'- cCGuGCGUCGAGAuccaGCCCGGagcCACCUu -3' miRNA: 3'- -GCcCGCAGUUCUug--CGGGUC---GUGGAc -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 48771 | 0.67 | 0.467293 |
Target: 5'- uGGGCcgguuauuacUCAGGAGCGCCCgaaaGGCGCUc- -3' miRNA: 3'- gCCCGc---------AGUUCUUGCGGG----UCGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 16823 | 0.67 | 0.467293 |
Target: 5'- -aGGCGUCGucucAgGUUCAGCACCUGg -3' miRNA: 3'- gcCCGCAGUucu-UgCGGGUCGUGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 2636 | 0.67 | 0.457375 |
Target: 5'- uGGGCGUCGuuaAGcGgGCCaGGUGCCUGc -3' miRNA: 3'- gCCCGCAGU---UCuUgCGGgUCGUGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 6288 | 0.67 | 0.457375 |
Target: 5'- uCGGGCu----GGACGCCUGGCacgACCUGg -3' miRNA: 3'- -GCCCGcaguuCUUGCGGGUCG---UGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 41814 | 0.69 | 0.382422 |
Target: 5'- aGGGCGUCGAuGAugGCUCc-CGCCa- -3' miRNA: 3'- gCCCGCAGUU-CUugCGGGucGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 25048 | 0.69 | 0.373642 |
Target: 5'- gCGGGCuUCGGccGCGCUggUAGCACCUGu -3' miRNA: 3'- -GCCCGcAGUUcuUGCGG--GUCGUGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 31403 | 0.69 | 0.373642 |
Target: 5'- aGGGUGUCGGccagcgugguGAACGCUggcguCAGCGCCg- -3' miRNA: 3'- gCCCGCAGUU----------CUUGCGG-----GUCGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 34945 | 0.69 | 0.365001 |
Target: 5'- gGuGGCGUCuccaAAGAGCGCCCAcCGCUg- -3' miRNA: 3'- gC-CCGCAG----UUCUUGCGGGUcGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 22231 | 0.69 | 0.348136 |
Target: 5'- aCGGGUGgaUCAAGAACgaccgccccgGCCCAGCgaugGCCg- -3' miRNA: 3'- -GCCCGC--AGUUCUUG----------CGGGUCG----UGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 32010 | 0.71 | 0.279255 |
Target: 5'- uGGGUGUCGAGGAucggcuCGCCguugAGCACCUu -3' miRNA: 3'- gCCCGCAGUUCUU------GCGGg---UCGUGGAc -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 16239 | 0.72 | 0.239649 |
Target: 5'- gCGGaGCGUCGAGGugGUCagCAGUACCg- -3' miRNA: 3'- -GCC-CGCAGUUCUugCGG--GUCGUGGac -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 13135 | 0.75 | 0.151106 |
Target: 5'- uGGGUucgcggaggucuccGaCGAGuACGCCCAGCGCCUGa -3' miRNA: 3'- gCCCG--------------CaGUUCuUGCGGGUCGUGGAC- -5' |
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7221 | 5' | -57.9 | NC_001900.1 | + | 11002 | 0.77 | 0.112359 |
Target: 5'- uCGGcGCuGUCAAGAACGaCCCAGCuCCUa -3' miRNA: 3'- -GCC-CG-CAGUUCUUGC-GGGUCGuGGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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