Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7222 | 5' | -55.8 | NC_001900.1 | + | 7657 | 0.67 | 0.588163 |
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Target: 5'- -aCCaaGGGggUUGGgggCGCAGGGCGu -3' miRNA: 3'- gaGGacCUCuuGACCa--GCGUCCUGCu -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 16977 | 0.68 | 0.523314 |
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Target: 5'- --gCUGGAGccgGugUGGUCGCAcucggcauGGACGGc -3' miRNA: 3'- gagGACCUC---UugACCAGCGU--------CCUGCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 24582 | 0.69 | 0.491905 |
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Target: 5'- uCUCCUcG-GAGCUGG-CGguGGugGAc -3' miRNA: 3'- -GAGGAcCuCUUGACCaGCguCCugCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 13879 | 0.69 | 0.481628 |
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Target: 5'- cCUCaUGGAGGGCggUGG-CGuCGGGGCGAa -3' miRNA: 3'- -GAGgACCUCUUG--ACCaGC-GUCCUGCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 28600 | 0.71 | 0.376414 |
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Target: 5'- aUCUUGGGGAACUgcagcucaGGgCGCAGGAaCGGg -3' miRNA: 3'- gAGGACCUCUUGA--------CCaGCGUCCU-GCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 18177 | 0.71 | 0.376414 |
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Target: 5'- -aUCUGGAGAuGCUGGg-GCGGGAgGAg -3' miRNA: 3'- gaGGACCUCU-UGACCagCGUCCUgCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 23704 | 0.72 | 0.337247 |
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Target: 5'- gCUCCUGGGGAgccgggaccaucuucACguUGGUCGCuGGcACGGu -3' miRNA: 3'- -GAGGACCUCU---------------UG--ACCAGCGuCC-UGCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 47689 | 0.74 | 0.253331 |
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Target: 5'- aUCCgacgGuGAGuGCUGGUCGCGGGcgGCGAc -3' miRNA: 3'- gAGGa---C-CUCuUGACCAGCGUCC--UGCU- -5' |
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| 7222 | 5' | -55.8 | NC_001900.1 | + | 13272 | 1.08 | 0.000863 |
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Target: 5'- gCUCCUGGAGAACUGGUCGCAGGACGAc -3' miRNA: 3'- -GAGGACCUCUUGACCAGCGUCCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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