Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7223 | 5' | -56.7 | NC_001900.1 | + | 11267 | 0.66 | 0.577744 |
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Target: 5'- uGUCUUCGaCGuGGAGAacUgGCCCGgAAa -3' miRNA: 3'- -CAGAAGCgGCuCCUCU--AgUGGGCgUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 11725 | 0.67 | 0.523412 |
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Target: 5'- --gUUCGUCgGAGGAGuUCGCCCaGCGGa -3' miRNA: 3'- cagAAGCGG-CUCCUCuAGUGGG-CGUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 45257 | 0.67 | 0.523412 |
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Target: 5'- uUCUUgacCGCCGucGAGGUCACgccgaCCGCGAa -3' miRNA: 3'- cAGAA---GCGGCucCUCUAGUG-----GGCGUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 22884 | 0.67 | 0.502215 |
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Target: 5'- ---aUCGCCGAGcGAGAccUCGCUgGCGg -3' miRNA: 3'- cagaAGCGGCUC-CUCU--AGUGGgCGUu -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 33002 | 0.68 | 0.471152 |
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Target: 5'- cUCUUCGCCGccAGGGGcuuccugCGCCUGCu- -3' miRNA: 3'- cAGAAGCGGC--UCCUCua-----GUGGGCGuu -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 15498 | 0.69 | 0.412156 |
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Target: 5'- aUCUUC-CCgGAGGAGAgcuuCCCGCAGg -3' miRNA: 3'- cAGAAGcGG-CUCCUCUagu-GGGCGUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 45706 | 0.7 | 0.366631 |
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Target: 5'- gGUCUccaGuUCGAGGAacaGGUCGCCCGCAGg -3' miRNA: 3'- -CAGAag-C-GGCUCCU---CUAGUGGGCGUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 20391 | 0.7 | 0.357951 |
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Target: 5'- ---aUCGCCGAGGAGGgcaUCACCUuccaggugaGCAAc -3' miRNA: 3'- cagaAGCGGCUCCUCU---AGUGGG---------CGUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 28053 | 0.7 | 0.341027 |
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Target: 5'- ---cUCGCCGGGGAacguguaGUCACCCGUGAa -3' miRNA: 3'- cagaAGCGGCUCCUc------UAGUGGGCGUU- -5' |
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| 7223 | 5' | -56.7 | NC_001900.1 | + | 12409 | 1.05 | 0.001012 |
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Target: 5'- gGUCUUCGCCGAGGAGAUCACCCGCAAc -3' miRNA: 3'- -CAGAAGCGGCUCCUCUAGUGGGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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