Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7227 | 3' | -51 | NC_001900.1 | + | 44519 | 0.66 | 0.887872 |
Target: 5'- cGCuGGCCGuuGUACGgaccUGACCAcGUCc -3' miRNA: 3'- -CGuUCGGCuuCAUGU----ACUGGUaCGGc -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 46136 | 0.66 | 0.887872 |
Target: 5'- ---cGCCGAgcuGGUcgAUGACCAgGCCGu -3' miRNA: 3'- cguuCGGCU---UCAugUACUGGUaCGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 16795 | 0.66 | 0.88009 |
Target: 5'- cGUucuGCCGA---ACAUGACCAacucGCCGa -3' miRNA: 3'- -CGuu-CGGCUucaUGUACUGGUa---CGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 48259 | 0.66 | 0.88009 |
Target: 5'- aGCGGGCCGuuuuGGUAUcgGcAUCgAUGCCc -3' miRNA: 3'- -CGUUCGGCu---UCAUGuaC-UGG-UACGGc -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 47380 | 0.66 | 0.872032 |
Target: 5'- --cAGCgCGGAGUagaccggcgggACGUGGCCcuUGCCGa -3' miRNA: 3'- cguUCG-GCUUCA-----------UGUACUGGu-ACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 26017 | 0.66 | 0.872032 |
Target: 5'- ---cGcCCGGAGUacguuguagGCGUGccGCCGUGCCGu -3' miRNA: 3'- cguuC-GGCUUCA---------UGUAC--UGGUACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 46786 | 0.66 | 0.872032 |
Target: 5'- cGCAGGCCGAucccuugGCGUuGAUCAgGUCGa -3' miRNA: 3'- -CGUUCGGCUuca----UGUA-CUGGUaCGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 37527 | 0.66 | 0.872032 |
Target: 5'- cGCcGGCCGGAGUGCcuucgguaGUuGCgGUGUCGg -3' miRNA: 3'- -CGuUCGGCUUCAUG--------UAcUGgUACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 42428 | 0.67 | 0.855114 |
Target: 5'- cCAGGcCCGAGGggaucggGC-UGGCCcGUGCCGu -3' miRNA: 3'- cGUUC-GGCUUCa------UGuACUGG-UACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 7674 | 0.67 | 0.855114 |
Target: 5'- cGCAGGgCGucaccGUACGUGACCuagGCUc -3' miRNA: 3'- -CGUUCgGCuu---CAUGUACUGGua-CGGc -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 31205 | 0.67 | 0.855114 |
Target: 5'- -uGGGCCGAcg-ACcuccuucuUGGCCGUGCCGu -3' miRNA: 3'- cgUUCGGCUucaUGu-------ACUGGUACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 2521 | 0.67 | 0.846272 |
Target: 5'- aCGAGCUGggGaGC-UGAUCAUGgCCa -3' miRNA: 3'- cGUUCGGCuuCaUGuACUGGUAC-GGc -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 16011 | 0.67 | 0.846272 |
Target: 5'- cCGGGCCGAGcuGUACGcGACCGuacUGCgGa -3' miRNA: 3'- cGUUCGGCUU--CAUGUaCUGGU---ACGgC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 28540 | 0.67 | 0.840849 |
Target: 5'- aGCAGGUucaCGAAcaGCGUGACCAgcaagcaccacuugGCCGg -3' miRNA: 3'- -CGUUCG---GCUUcaUGUACUGGUa-------------CGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 31453 | 0.67 | 0.840849 |
Target: 5'- aGCGAGCUG-AGUGCccagagucccgagGACguUGCCGa -3' miRNA: 3'- -CGUUCGGCuUCAUGua-----------CUGguACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 18192 | 0.67 | 0.837186 |
Target: 5'- gGCGGGagGAGGUGCAUGAggcgcucgaCGUGCUGa -3' miRNA: 3'- -CGUUCggCUUCAUGUACUg--------GUACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 37735 | 0.67 | 0.837186 |
Target: 5'- cGCAA-CCGgcGUAaccGGCCAUGUCGg -3' miRNA: 3'- -CGUUcGGCuuCAUguaCUGGUACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 41310 | 0.67 | 0.827866 |
Target: 5'- -aGGGuuGggGcACAUGACCGuuUGUCGu -3' miRNA: 3'- cgUUCggCuuCaUGUACUGGU--ACGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 6029 | 0.67 | 0.827866 |
Target: 5'- cGCGAGCUGGAGcgAguUGuCCGcGCCGc -3' miRNA: 3'- -CGUUCGGCUUCa-UguACuGGUaCGGC- -5' |
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7227 | 3' | -51 | NC_001900.1 | + | 46874 | 0.68 | 0.818323 |
Target: 5'- uGguGGCCGAgaggcgGGUgaucACGUGGCCAUcucgGCCa -3' miRNA: 3'- -CguUCGGCU------UCA----UGUACUGGUA----CGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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