Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7228 | 3' | -61.8 | NC_001900.1 | + | 32291 | 0.66 | 0.385951 |
Target: 5'- aACGGcccgaGcCGCUucACGCGCCuucuuCCGGGGGa -3' miRNA: 3'- gUGCCa----C-GCGG--UGCGUGGu----GGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 16021 | 0.66 | 0.37738 |
Target: 5'- gGCGGUG-GCCAgcaGCACCAgucUCGGGc- -3' miRNA: 3'- gUGCCACgCGGUg--CGUGGU---GGCCCuc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 25046 | 0.66 | 0.37653 |
Target: 5'- gCGCGG-GCuucgGCCGCGCugguagcaccuguGCCGCCGuuGGGGa -3' miRNA: 3'- -GUGCCaCG----CGGUGCG-------------UGGUGGC--CCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 1176 | 0.66 | 0.360626 |
Target: 5'- gGCGGUGgaGCCGCcggaaGCgGCCGGGGc -3' miRNA: 3'- gUGCCACg-CGGUGcg---UGgUGGCCCUc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 28022 | 0.66 | 0.352445 |
Target: 5'- gGCuGGUGC-CCAgGaACCACgGGGAGu -3' miRNA: 3'- gUG-CCACGcGGUgCgUGGUGgCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 30923 | 0.66 | 0.339632 |
Target: 5'- gGCGacUGCGCCgacgagucugccaggACGCcaACCAgCCGGGAGa -3' miRNA: 3'- gUGCc-ACGCGG---------------UGCG--UGGU-GGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 16822 | 0.66 | 0.336482 |
Target: 5'- gAUGGU-CGUCACGggcaACCACCGGGc- -3' miRNA: 3'- gUGCCAcGCGGUGCg---UGGUGGCCCuc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 18881 | 0.66 | 0.336482 |
Target: 5'- gACGGUGCGaucuCCAagGcCAUCACCGaGGGGa -3' miRNA: 3'- gUGCCACGC----GGUg-C-GUGGUGGC-CCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 3173 | 0.67 | 0.3287 |
Target: 5'- aGCGGuUGcCGCUACcgacuGCucccCCGCCGGGGGu -3' miRNA: 3'- gUGCC-AC-GCGGUG-----CGu---GGUGGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 47304 | 0.67 | 0.327929 |
Target: 5'- aCGCGGUcGCGuCCGgGCAggagaacccuugcCCACUGGGcAGa -3' miRNA: 3'- -GUGCCA-CGC-GGUgCGU-------------GGUGGCCC-UC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 21237 | 0.67 | 0.321052 |
Target: 5'- uCGCGGaacugGCuaCAacggaGCGCCugCGGGAGc -3' miRNA: 3'- -GUGCCa----CGcgGUg----CGUGGugGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 3024 | 0.67 | 0.313538 |
Target: 5'- uCAUGGUugccauGCGCCGCGCucgGCgGCUGGGc- -3' miRNA: 3'- -GUGCCA------CGCGGUGCG---UGgUGGCCCuc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 10261 | 0.67 | 0.306158 |
Target: 5'- gGCGaUGCGCCugGC-CUACCGGc-- -3' miRNA: 3'- gUGCcACGCGGugCGuGGUGGCCcuc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 1839 | 0.68 | 0.277972 |
Target: 5'- aGCaGUGUGCUGCuGCACaACUGGGAGc -3' miRNA: 3'- gUGcCACGCGGUG-CGUGgUGGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 40735 | 0.69 | 0.237831 |
Target: 5'- aGCGGUcagcgaugucgucaGCGCCGCgaGCGCCGCCGuGAa -3' miRNA: 3'- gUGCCA--------------CGCGGUG--CGUGGUGGCcCUc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 17561 | 0.69 | 0.224431 |
Target: 5'- cUACGGcGUGaaccccgccaccaucUCGCGCACCGuCCGGGGGa -3' miRNA: 3'- -GUGCCaCGC---------------GGUGCGUGGU-GGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 21567 | 0.7 | 0.200583 |
Target: 5'- gACGGUGCGCCGCuaCAUCGCCGa--- -3' miRNA: 3'- gUGCCACGCGGUGc-GUGGUGGCccuc -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 40308 | 0.72 | 0.154468 |
Target: 5'- gCGCGGaacGCGaCCACGUcgUACCGGGAGa -3' miRNA: 3'- -GUGCCa--CGC-GGUGCGugGUGGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 16213 | 0.72 | 0.13887 |
Target: 5'- aACGGcUGauCCACGCuugacaGCCACCGGGGGc -3' miRNA: 3'- gUGCC-ACgcGGUGCG------UGGUGGCCCUC- -5' |
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7228 | 3' | -61.8 | NC_001900.1 | + | 24096 | 0.72 | 0.131625 |
Target: 5'- uGCGGcUGCGCCGC-CGCCACCaGGuGc -3' miRNA: 3'- gUGCC-ACGCGGUGcGUGGUGGcCCuC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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