Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7231 | 5' | -62 | NC_001900.1 | + | 7856 | 1.07 | 0.000216 |
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Target: 5'- cCGCUGCACCAGCAGGUGCUGGGCGCGc -3' miRNA: 3'- -GCGACGUGGUCGUCCACGACCCGCGC- -5' |
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| 7231 | 5' | -62 | NC_001900.1 | + | 15342 | 0.75 | 0.07306 |
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Target: 5'- aCGCUGCACCAGCuccuggcguuccAGGcgcucuaccagacgUGCUGGGC-CGa -3' miRNA: 3'- -GCGACGUGGUCG------------UCC--------------ACGACCCGcGC- -5' |
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| 7231 | 5' | -62 | NC_001900.1 | + | 35256 | 0.74 | 0.089614 |
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Target: 5'- cCGCU-CAcCCAGCAguugaacaGGUGCUGGGCGUu -3' miRNA: 3'- -GCGAcGU-GGUCGU--------CCACGACCCGCGc -5' |
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| 7231 | 5' | -62 | NC_001900.1 | + | 42557 | 0.7 | 0.172369 |
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Target: 5'- gGCUG-GCCAGCGGGccgaGCUGGGggacCGCGc -3' miRNA: 3'- gCGACgUGGUCGUCCa---CGACCC----GCGC- -5' |
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| 7231 | 5' | -62 | NC_001900.1 | + | 35891 | 0.67 | 0.259881 |
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Target: 5'- aCGCUuccaacCGCCAGCcgcgaucAGGcGCUGGGCGUc -3' miRNA: 3'- -GCGAc-----GUGGUCG-------UCCaCGACCCGCGc -5' |
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| 7231 | 5' | -62 | NC_001900.1 | + | 33381 | 0.67 | 0.29479 |
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Target: 5'- uGCUGCGCuucuuCAGCAGGaGCggugucgaccGGCGCGc -3' miRNA: 3'- gCGACGUG-----GUCGUCCaCGac--------CCGCGC- -5' |
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| 7231 | 5' | -62 | NC_001900.1 | + | 16957 | 0.67 | 0.29479 |
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Target: 5'- uCGCUGCugUAGCGuucGGcGCUGGaGC-CGg -3' miRNA: 3'- -GCGACGugGUCGU---CCaCGACC-CGcGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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