Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7232 | 3' | -59.4 | NC_001900.1 | + | 34153 | 0.69 | 0.301372 |
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Target: 5'- cCGAGCCUGAucaggcccuggauuCGCCaGGAGUCCugguAGAGGg -3' miRNA: 3'- aGCUCGGGCU--------------GUGG-CCUCGGG----UCUUC- -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 28012 | 0.69 | 0.296271 |
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Target: 5'- -gGAGCCCucGgGCUGGuGCCCAGGAa -3' miRNA: 3'- agCUCGGGc-UgUGGCCuCGGGUCUUc -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 13135 | 0.7 | 0.289102 |
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Target: 5'- aCGAGCcgCCGAUcCCGGAGuuCCUGGGAGg -3' miRNA: 3'- aGCUCG--GGCUGuGGCCUC--GGGUCUUC- -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 16657 | 0.71 | 0.236605 |
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Target: 5'- gUCGGGCuCCGACGCCGacaaGGCCaaGGAAGu -3' miRNA: 3'- -AGCUCG-GGCUGUGGCc---UCGGg-UCUUC- -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 23829 | 0.71 | 0.224794 |
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Target: 5'- aUCGGcGCuggcuuCCGGCGCUGGGGCUgGGAAGg -3' miRNA: 3'- -AGCU-CG------GGCUGUGGCCUCGGgUCUUC- -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 6695 | 0.71 | 0.224794 |
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Target: 5'- cUCGAGCgaUCGACGCgGGcAGUCCGGGAa -3' miRNA: 3'- -AGCUCG--GGCUGUGgCC-UCGGGUCUUc -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 44081 | 0.72 | 0.212935 |
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Target: 5'- -gGAGCCCGACgagcaugACCGGGGugaauCCCAGGGc -3' miRNA: 3'- agCUCGGGCUG-------UGGCCUC-----GGGUCUUc -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 2578 | 0.78 | 0.075914 |
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Target: 5'- gCGuAGCCCGACGgCGGgaacucaaGGCCCGGAGGa -3' miRNA: 3'- aGC-UCGGGCUGUgGCC--------UCGGGUCUUC- -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 13833 | 0.79 | 0.064086 |
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Target: 5'- aUGAGCCuccuCGAUACCGGAGCCCGGu-- -3' miRNA: 3'- aGCUCGG----GCUGUGGCCUCGGGUCuuc -5' |
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| 7232 | 3' | -59.4 | NC_001900.1 | + | 7463 | 1.08 | 0.000431 |
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Target: 5'- aUCGAGCCCGACACCGGAGCCCAGAAGc -3' miRNA: 3'- -AGCUCGGGCUGUGGCCUCGGGUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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