Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7232 | 5' | -53.8 | NC_001900.1 | + | 30401 | 0.66 | 0.737212 |
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Target: 5'- cGCCUGCAGcGCcGcACGGCCGGc--- -3' miRNA: 3'- cUGGACGUU-CGuCuUGCCGGUCaagu -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 36416 | 0.66 | 0.704112 |
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Target: 5'- gGACCUGCuuGCGGAcguagaucACGGCC---UCAc -3' miRNA: 3'- -CUGGACGuuCGUCU--------UGCCGGucaAGU- -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 43094 | 0.66 | 0.704112 |
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Target: 5'- aGCC-GCuuGCGGccUGGCCGGUUCAg -3' miRNA: 3'- cUGGaCGuuCGUCuuGCCGGUCAAGU- -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 17491 | 0.69 | 0.512811 |
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Target: 5'- -gUCUGCAGGC-GAugGGUCAGcUCGg -3' miRNA: 3'- cuGGACGUUCGuCUugCCGGUCaAGU- -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 19598 | 0.71 | 0.453678 |
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Target: 5'- cGGCC-GCGAGCAGGGCGGgcucaaccccaccgaCCAGUaCAu -3' miRNA: 3'- -CUGGaCGUUCGUCUUGCC---------------GGUCAaGU- -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 31584 | 0.71 | 0.439476 |
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Target: 5'- gGACCUcacgcucggugaGCuucuGGCGGuuCGGCCGGUUCGg -3' miRNA: 3'- -CUGGA------------CGu---UCGUCuuGCCGGUCAAGU- -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 13296 | 0.73 | 0.345992 |
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Target: 5'- cGACCUG-GAGCAGuacgucGGCGGCguGUUCAc -3' miRNA: 3'- -CUGGACgUUCGUC------UUGCCGguCAAGU- -5' |
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| 7232 | 5' | -53.8 | NC_001900.1 | + | 7497 | 1.09 | 0.00104 |
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Target: 5'- uGACCUGCAAGCAGAACGGCCAGUUCAa -3' miRNA: 3'- -CUGGACGUUCGUCUUGCCGGUCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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