Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7235 | 3' | -51 | NC_001900.1 | + | 15221 | 0.66 | 0.861472 |
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Target: 5'- -cGAGAUCaCCaccgagGAUCCGa-UCGACUACg -3' miRNA: 3'- uaCUCUAG-GG------CUAGGCcaAGUUGAUG- -5' |
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| 7235 | 3' | -51 | NC_001900.1 | + | 14253 | 0.67 | 0.852763 |
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Target: 5'- -cGAGAUCCUGAgCCGGUgu-GCccGCg -3' miRNA: 3'- uaCUCUAGGGCUaGGCCAaguUGa-UG- -5' |
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| 7235 | 3' | -51 | NC_001900.1 | + | 43230 | 0.67 | 0.843801 |
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Target: 5'- -gGAGGUCgUGGUCCGGgcCAGCc-- -3' miRNA: 3'- uaCUCUAGgGCUAGGCCaaGUUGaug -5' |
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| 7235 | 3' | -51 | NC_001900.1 | + | 30685 | 0.7 | 0.698016 |
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Target: 5'- aGUG-GAUCCCGAggaagCCGGUcacagcguucuUCAcGCUGCu -3' miRNA: 3'- -UACuCUAGGGCUa----GGCCA-----------AGU-UGAUG- -5' |
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| 7235 | 3' | -51 | NC_001900.1 | + | 39265 | 0.72 | 0.526877 |
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Target: 5'- -cGAGAgCCgGGUCCGGUUCGGaaGCg -3' miRNA: 3'- uaCUCUaGGgCUAGGCCAAGUUgaUG- -5' |
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| 7235 | 3' | -51 | NC_001900.1 | + | 7387 | 0.73 | 0.483277 |
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Target: 5'- -cGAGAUCCCGcgCCGGaucguguucUUCAACg-- -3' miRNA: 3'- uaCUCUAGGGCuaGGCC---------AAGUUGaug -5' |
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| 7235 | 3' | -51 | NC_001900.1 | + | 6758 | 1.09 | 0.002134 |
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Target: 5'- gAUGAGAUCCCGAUCCGGUUCAACUACa -3' miRNA: 3'- -UACUCUAGGGCUAGGCCAAGUUGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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