Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7237 | 3' | -56.3 | NC_001900.1 | + | 21850 | 0.66 | 0.634157 |
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Target: 5'- cGAUUCUGAgccacuuuccUGGGcCUCCCGguaGAGGAg -3' miRNA: 3'- -CUAGGACU----------ACCCcGAGGGCug-CUUCU- -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 9580 | 0.66 | 0.612094 |
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Target: 5'- uGAUCCgGGUGGaGCcCCCGACGcgcauguacgccGAGAu -3' miRNA: 3'- -CUAGGaCUACCcCGaGGGCUGC------------UUCU- -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 27414 | 0.66 | 0.612094 |
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Target: 5'- --aCC-GAUGGGGCgcaucagcgCCuCGACGGAGu -3' miRNA: 3'- cuaGGaCUACCCCGa--------GG-GCUGCUUCu -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 20807 | 0.66 | 0.590101 |
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Target: 5'- --aCCUGAagggucugUGGGGCaucgacCCCGuCGAAGGu -3' miRNA: 3'- cuaGGACU--------ACCCCGa-----GGGCuGCUUCU- -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 14255 | 0.67 | 0.579153 |
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Target: 5'- aGAUCCUGAgccGGUgugCCCG-CGAAGGc -3' miRNA: 3'- -CUAGGACUaccCCGa--GGGCuGCUUCU- -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 15179 | 0.67 | 0.546607 |
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Target: 5'- cGAUUCcGAggucaaGGGCUCCUGGCaGAAGAa -3' miRNA: 3'- -CUAGGaCUac----CCCGAGGGCUG-CUUCU- -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 23134 | 0.69 | 0.463248 |
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Target: 5'- --cCCUGAccGGGCgcagCCCGgGCGAGGAc -3' miRNA: 3'- cuaGGACUacCCCGa---GGGC-UGCUUCU- -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 30850 | 0.69 | 0.424122 |
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Target: 5'- uGAUCUUGccGGGGagcugUCCGACGAAGu -3' miRNA: 3'- -CUAGGACuaCCCCga---GGGCUGCUUCu -5' |
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| 7237 | 3' | -56.3 | NC_001900.1 | + | 5654 | 1.09 | 0.000707 |
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Target: 5'- cGAUCCUGAUGGGGCUCCCGACGAAGAu -3' miRNA: 3'- -CUAGGACUACCCCGAGGGCUGCUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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