Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7237 | 5' | -52.6 | NC_001900.1 | + | 37183 | 0.66 | 0.868753 |
Target: 5'- gUUCUUCuUCGCGGacUCGGC--GAUCGa -3' miRNA: 3'- gGAGAAGuAGCGCC--AGCUGguCUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 34698 | 0.66 | 0.860577 |
Target: 5'- -gUCcaCAUCGCcGUCGACCucuucGAUCGa -3' miRNA: 3'- ggAGaaGUAGCGcCAGCUGGu----CUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 28219 | 0.66 | 0.852157 |
Target: 5'- gCCUCgUCcUUGCGGaCGGCCucGUCGg -3' miRNA: 3'- -GGAGaAGuAGCGCCaGCUGGucUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 27941 | 0.66 | 0.852157 |
Target: 5'- aCUCUcccaGUC-CGGUCGACUcaucagaagaAGAUCGg -3' miRNA: 3'- gGAGAag--UAGcGCCAGCUGG----------UCUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 48711 | 0.66 | 0.846991 |
Target: 5'- gCCgcgCUUCAggCGCGG-CGcuagauccggcgguuGCCGGAUCa -3' miRNA: 3'- -GGa--GAAGUa-GCGCCaGC---------------UGGUCUAGc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 36275 | 0.67 | 0.804789 |
Target: 5'- --cCUUCAUCGCcagcggcaggauGUCGACCAGGUa- -3' miRNA: 3'- ggaGAAGUAGCGc-----------CAGCUGGUCUAgc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 26250 | 0.67 | 0.797022 |
Target: 5'- cCUUCUUC-UCGCGcUCGGCCggcgGGGUCc -3' miRNA: 3'- -GGAGAAGuAGCGCcAGCUGG----UCUAGc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 18025 | 0.68 | 0.777127 |
Target: 5'- gCCgg-UCAUCGUgccGGUgGuCCAGAUCGu -3' miRNA: 3'- -GGagaAGUAGCG---CCAgCuGGUCUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 34894 | 0.68 | 0.777127 |
Target: 5'- aCCUCUUgAUCGUGuaGUCGACCcGcgCc -3' miRNA: 3'- -GGAGAAgUAGCGC--CAGCUGGuCuaGc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 43364 | 0.68 | 0.756619 |
Target: 5'- aCUCUUCgAUC-UGGUCGACCgcgaugaagAGGUCa -3' miRNA: 3'- gGAGAAG-UAGcGCCAGCUGG---------UCUAGc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 35631 | 0.69 | 0.714143 |
Target: 5'- aCCgcgUCGgccUCGauGUCGAUCAGAUCGg -3' miRNA: 3'- -GGagaAGU---AGCgcCAGCUGGUCUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 3676 | 0.69 | 0.714143 |
Target: 5'- aCUUCUUCGaUGCGuUCGACCAGcuccUCGg -3' miRNA: 3'- -GGAGAAGUaGCGCcAGCUGGUCu---AGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 33189 | 0.69 | 0.703288 |
Target: 5'- aCCUCgucgggaUCAaCGCccaGGUCGACCAGAg-- -3' miRNA: 3'- -GGAGa------AGUaGCG---CCAGCUGGUCUagc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 3787 | 0.69 | 0.692362 |
Target: 5'- gCgUCgUCAccuUCGCGGUCGGCguGAccUCGa -3' miRNA: 3'- -GgAGaAGU---AGCGCCAGCUGguCU--AGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 30583 | 0.7 | 0.625956 |
Target: 5'- ------gGUCGaUGGUCGACCAGAUCu -3' miRNA: 3'- ggagaagUAGC-GCCAGCUGGUCUAGc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 33387 | 0.71 | 0.570658 |
Target: 5'- gCUUCUUCAgcaggaGCGGugUCGACCGGcgCGc -3' miRNA: 3'- -GGAGAAGUag----CGCC--AGCUGGUCuaGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 6841 | 0.72 | 0.506048 |
Target: 5'- aCUCgcagggagcCAUCGUGGUUGGCCAGGUg- -3' miRNA: 3'- gGAGaa-------GUAGCGCCAGCUGGUCUAgc -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 4737 | 0.73 | 0.49557 |
Target: 5'- gCCg--UCGUCGUGGUCGACgGGGagcuUCGa -3' miRNA: 3'- -GGagaAGUAGCGCCAGCUGgUCU----AGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 38110 | 0.73 | 0.474917 |
Target: 5'- gCCUCggCGUUGCgcgucaGGUCGACCAcGUCGu -3' miRNA: 3'- -GGAGaaGUAGCG------CCAGCUGGUcUAGC- -5' |
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7237 | 5' | -52.6 | NC_001900.1 | + | 5693 | 1.12 | 0.001238 |
Target: 5'- aCCUCUUCAUCGCGGUCGACCAGAUCGa -3' miRNA: 3'- -GGAGAAGUAGCGCCAGCUGGUCUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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