Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7238 | 3' | -54 | NC_001900.1 | + | 44871 | 0.66 | 0.751531 |
Target: 5'- cGAGCCcacgaccucucGGAUCuCAuCcAGCGAUGGGCa -3' miRNA: 3'- aCUUGG-----------UCUAG-GUcGcUCGCUACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 26678 | 0.66 | 0.751531 |
Target: 5'- aGGACCGGAUCCc-CGAGagg-GGGCc -3' miRNA: 3'- aCUUGGUCUAGGucGCUCgcuaCCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 39702 | 0.66 | 0.740857 |
Target: 5'- gGAA-CAGAUCCAGCaGAgccagcGCGAUGaGAUc -3' miRNA: 3'- aCUUgGUCUAGGUCG-CU------CGCUAC-CUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 15908 | 0.66 | 0.730068 |
Target: 5'- cUGGGCCAGggCCAGa-AGUgguucgcccuGAUGGACc -3' miRNA: 3'- -ACUUGGUCuaGGUCgcUCG----------CUACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 665 | 0.66 | 0.708193 |
Target: 5'- cGcGCCGGAUCCGGUGAaaaucCGGUGuGAUu -3' miRNA: 3'- aCuUGGUCUAGGUCGCUc----GCUAC-CUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 23236 | 0.67 | 0.690462 |
Target: 5'- gUGGACCAGccugcgGUUCGGUGAGCuGAUcgagauccgccgcaaGGACa -3' miRNA: 3'- -ACUUGGUC------UAGGUCGCUCG-CUA---------------CCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 43492 | 0.67 | 0.686004 |
Target: 5'- gUGAACCAcGAagUAGuCGGGCGuggGGACc -3' miRNA: 3'- -ACUUGGU-CUagGUC-GCUCGCua-CCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 39889 | 0.67 | 0.674823 |
Target: 5'- cGGAUCAGcgaucuccuccuGUCCGGgGAGCGGgcugucaucGGACg -3' miRNA: 3'- aCUUGGUC------------UAGGUCgCUCGCUa--------CCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 47503 | 0.67 | 0.674823 |
Target: 5'- cGAGuCCAGGagcuugUCCAGgugcuuCGuGCGGUGGACc -3' miRNA: 3'- aCUU-GGUCU------AGGUC------GCuCGCUACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 44953 | 0.67 | 0.663598 |
Target: 5'- aUGAACCAacugCCAGCGgcAGCGGUucgGGAUc -3' miRNA: 3'- -ACUUGGUcua-GGUCGC--UCGCUA---CCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 7694 | 0.67 | 0.652344 |
Target: 5'- cGAagGCguGAUCCGGCGgcucaAGgGAUGGGg -3' miRNA: 3'- aCU--UGguCUAGGUCGC-----UCgCUACCUg -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 41769 | 0.68 | 0.629786 |
Target: 5'- ---cCCAGA-CCGGCG-GCGA-GGGCg -3' miRNA: 3'- acuuGGUCUaGGUCGCuCGCUaCCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 10404 | 0.68 | 0.618506 |
Target: 5'- aGGGCguGAUCCcgcGUGAGCGGgcucgcaaGGACa -3' miRNA: 3'- aCUUGguCUAGGu--CGCUCGCUa-------CCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 1140 | 0.68 | 0.595991 |
Target: 5'- gGAGCCGGggCaGGCG-GCGuggGUGGACu -3' miRNA: 3'- aCUUGGUCuaGgUCGCuCGC---UACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 10906 | 0.69 | 0.573606 |
Target: 5'- --cACCGGAUCCAGC-AGUuccUGGACg -3' miRNA: 3'- acuUGGUCUAGGUCGcUCGcu-ACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 28264 | 0.69 | 0.562484 |
Target: 5'- cGGAUCAGGUCCGGgaGGGCuccGUGGAg -3' miRNA: 3'- aCUUGGUCUAGGUCg-CUCGc--UACCUg -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 41085 | 0.71 | 0.435657 |
Target: 5'- cGGGCCAGG-CCGGUGAccucGCGGccgUGGACc -3' miRNA: 3'- aCUUGGUCUaGGUCGCU----CGCU---ACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 39267 | 0.72 | 0.369694 |
Target: 5'- aGAGCCGGGUCCGGUucggaAGCGGgaccaccgGGACc -3' miRNA: 3'- aCUUGGUCUAGGUCGc----UCGCUa-------CCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 18317 | 0.72 | 0.369694 |
Target: 5'- cUGGACacgCAGAUCCuGGCGaAGCuGGUGGACc -3' miRNA: 3'- -ACUUG---GUCUAGG-UCGC-UCG-CUACCUG- -5' |
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7238 | 3' | -54 | NC_001900.1 | + | 9878 | 0.74 | 0.306204 |
Target: 5'- cUGGGCgCGGGauugcaucgagaugcUCCAGCGAGCGAUcGACu -3' miRNA: 3'- -ACUUG-GUCU---------------AGGUCGCUCGCUAcCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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