miRNA: 3'- -GCCGCGUGGCUCGACCGGCCGGCUAG- -5'
miRNA: 3'- gCCGCGUgGCU-CGaCCGGCCGGCua--G- -5'
miRNA: 3'- -GCCGCg--UGgCUCGACCGgCCGgCUAG- -5'
miRNA: 3'- gCCGCGu---GGCuCGACCGG----CC------GGCUAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7238 | 5' | -63.6 | NC_001900.1 | + | 5582 | 1.08 | 0.000176 |
|
Target: 5'- gCGGCGCACCGAGCUGGCCGGCCGAUCc -3' miRNA: 3'- -GCCGCGUGGCUCGACCGGCCGGCUAG- -5' |
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| 7238 | 5' | -63.6 | NC_001900.1 | + | 3383 | 0.66 | 0.266094 |
|
Target: 5'- aGGUGUGgUGAgGCcGGCCGGCCGugagCg -3' miRNA: 3'- gCCGCGUgGCU-CGaCCGGCCGGCua--G- -5' |
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| 7238 | 5' | -63.6 | NC_001900.1 | + | 1249 | 0.69 | 0.182402 |
|
Target: 5'- gCGGCGgcaGCgGAGCUGGC-GGCgGGUUc -3' miRNA: 3'- -GCCGCg--UGgCUCGACCGgCCGgCUAG- -5' |
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| 7238 | 5' | -63.6 | NC_001900.1 | + | 912 | 0.67 | 0.253352 |
|
Target: 5'- aGGCGCuuuuCCGcGCUGGCCuccaGGaacuccUCGAUCg -3' miRNA: 3'- gCCGCGu---GGCuCGACCGG----CC------GGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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