Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7242 | 3' | -53.3 | NC_001900.1 | + | 41105 | 0.66 | 0.749765 |
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Target: 5'- --gCCUCGaugCGGCCACCggccgCGGAGUa- -3' miRNA: 3'- cuaGGAGUa--GCUGGUGGa----GCUUCGac -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 26946 | 0.67 | 0.728248 |
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Target: 5'- -cUCCUUcUCGGcCCACCUCGcuGCg- -3' miRNA: 3'- cuAGGAGuAGCU-GGUGGAGCuuCGac -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 3152 | 0.67 | 0.706327 |
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Target: 5'- aGAUCCUCG-CGGCCA--UCGAAGUcuUGa -3' miRNA: 3'- -CUAGGAGUaGCUGGUggAGCUUCG--AC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 20357 | 0.67 | 0.695246 |
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Target: 5'- cGUCUUCuUCGACgGCCa-GGAGCUGc -3' miRNA: 3'- cUAGGAGuAGCUGgUGGagCUUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 45370 | 0.68 | 0.6841 |
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Target: 5'- -cUCCaCGUCGACC-CCgaGGAGCUGg -3' miRNA: 3'- cuAGGaGUAGCUGGuGGagCUUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 23901 | 0.68 | 0.6841 |
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Target: 5'- --gCCUCGUaccCGGCCcgaGCCUCugcGAGGCUGg -3' miRNA: 3'- cuaGGAGUA---GCUGG---UGGAG---CUUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 27053 | 0.68 | 0.672903 |
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Target: 5'- cGUCCUCAUCaGCCAgCUccCGGAGCc- -3' miRNA: 3'- cUAGGAGUAGcUGGUgGA--GCUUCGac -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 33232 | 0.68 | 0.639112 |
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Target: 5'- uGUCCUCGaccuccUUGACgGCCUCGAccacGGCUu -3' miRNA: 3'- cUAGGAGU------AGCUGgUGGAGCU----UCGAc -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 39791 | 0.66 | 0.781066 |
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Target: 5'- --gCCUCugGUCGGCgcucaGCCUCGuaguAGCUGg -3' miRNA: 3'- cuaGGAG--UAGCUGg----UGGAGCu---UCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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