Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7242 | 3' | -53.3 | NC_001900.1 | + | 33232 | 0.68 | 0.639112 |
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Target: 5'- uGUCCUCGaccuccUUGACgGCCUCGAccacGGCUu -3' miRNA: 3'- cUAGGAGU------AGCUGgUGGAGCU----UCGAc -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 3973 | 0.68 | 0.639112 |
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Target: 5'- --gUCUCA-CGGgCACCUCGAAGCc- -3' miRNA: 3'- cuaGGAGUaGCUgGUGGAGCUUCGac -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 24549 | 0.69 | 0.576088 |
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Target: 5'- cAUCCcggcacaggccgaguUCAUCGACgugauucuCCUCGGAGCUGg -3' miRNA: 3'- cUAGG---------------AGUAGCUGgu------GGAGCUUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 12770 | 0.7 | 0.560505 |
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Target: 5'- cAUCCUCAgCGACCACgUCGuggaGGGCa- -3' miRNA: 3'- cUAGGAGUaGCUGGUGgAGC----UUCGac -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 12937 | 0.7 | 0.538455 |
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Target: 5'- --aCCUCGUCGcaguCCGugUCGAGGCUGa -3' miRNA: 3'- cuaGGAGUAGCu---GGUggAGCUUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 45305 | 0.71 | 0.499572 |
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Target: 5'- --aCCUCAUCGAacgagcgccgcgaggUCACCUUG-AGCUGg -3' miRNA: 3'- cuaGGAGUAGCU---------------GGUGGAGCuUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 32804 | 0.72 | 0.453833 |
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Target: 5'- cGGUCCUCAUC--CCaACCUCGGuacuGCUGa -3' miRNA: 3'- -CUAGGAGUAGcuGG-UGGAGCUu---CGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 22699 | 0.74 | 0.317271 |
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Target: 5'- -cUCCUCcgCGAgCACCUgcUGAAGCUGc -3' miRNA: 3'- cuAGGAGuaGCUgGUGGA--GCUUCGAC- -5' |
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| 7242 | 3' | -53.3 | NC_001900.1 | + | 2888 | 1.08 | 0.001616 |
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Target: 5'- aGAUCCUCAUCGACCACCUCGAAGCUGc -3' miRNA: 3'- -CUAGGAGUAGCUGGUGGAGCUUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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