Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7242 | 5' | -57.1 | NC_001900.1 | + | 25916 | 0.7 | 0.327325 |
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Target: 5'- -aCGACGGUCUGGUCcUCGcCCGGgCUg -3' miRNA: 3'- gcGUUGCCGGACCAGuAGCuGGUC-GA- -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 3558 | 0.71 | 0.319424 |
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Target: 5'- gGUAACGGCCUGGUgCAUcCGGuCUcGCUg -3' miRNA: 3'- gCGUUGCCGGACCA-GUA-GCU-GGuCGA- -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 17266 | 0.71 | 0.282075 |
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Target: 5'- gGCucgGGCC-GGUCAucUCGACCGGCa -3' miRNA: 3'- gCGuugCCGGaCCAGU--AGCUGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 43752 | 0.72 | 0.275033 |
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Target: 5'- uGCAGuCGGCCUGGaugaggccgUCAcgcaCGGCCGGCa -3' miRNA: 3'- gCGUU-GCCGGACC---------AGUa---GCUGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 30819 | 0.72 | 0.248272 |
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Target: 5'- aGCcAgGaGCCUGcGUCAUCGAaCCAGCUc -3' miRNA: 3'- gCGuUgC-CGGAC-CAGUAGCU-GGUCGA- -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 24740 | 0.72 | 0.248272 |
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Target: 5'- cCGCAG-GGCCUGGUuaCAUCuucggacaGACCGGCg -3' miRNA: 3'- -GCGUUgCCGGACCA--GUAG--------CUGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 8091 | 0.77 | 0.113017 |
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Target: 5'- aCGCGAUGGCCgaGGUCAUCGAa-GGCa -3' miRNA: 3'- -GCGUUGCCGGa-CCAGUAGCUggUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 33608 | 0.81 | 0.067627 |
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Target: 5'- gGCAguGCGGCaaGGUCAUCGAUCGGCa -3' miRNA: 3'- gCGU--UGCCGgaCCAGUAGCUGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 2922 | 1.07 | 0.000767 |
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Target: 5'- gCGCAACGGCCUGGUCAUCGACCAGCUc -3' miRNA: 3'- -GCGUUGCCGGACCAGUAGCUGGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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