Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7243 | 5' | -55.3 | NC_001900.1 | + | 33022 | 0.66 | 0.662024 |
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Target: 5'- cCUG-CGCcuGCUGCGGaGCCcgagacuggUGCUGCu -3' miRNA: 3'- -GACaGCGu-UGACGCCaCGG---------ACGAUGu -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 5606 | 0.66 | 0.647437 |
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Target: 5'- gCUGUCGgAGCUGCgcugggcaggcaucGGUGCCgccUGgUACu -3' miRNA: 3'- -GACAGCgUUGACG--------------CCACGG---ACgAUGu -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 2640 | 0.67 | 0.561292 |
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Target: 5'- -cGUCGUuaAGCggGCcaGGUGCCUGCUcCAc -3' miRNA: 3'- gaCAGCG--UUGa-CG--CCACGGACGAuGU- -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 34026 | 0.68 | 0.496353 |
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Target: 5'- -cGgCGUAugUgcaGCGGUGCCUGCUucgGCGa -3' miRNA: 3'- gaCaGCGUugA---CGCCACGGACGA---UGU- -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 9449 | 0.71 | 0.366687 |
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Target: 5'- --aUCGCGugUGCGGUgcugucguacuucuGCCUGCUGa- -3' miRNA: 3'- gacAGCGUugACGCCA--------------CGGACGAUgu -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 25936 | 0.71 | 0.343354 |
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Target: 5'- -cGUCGC-GCUcaGCGGUGCCUuCUGCu -3' miRNA: 3'- gaCAGCGuUGA--CGCCACGGAcGAUGu -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 8919 | 0.74 | 0.233515 |
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Target: 5'- -aGUCGCGGCaGCGGU-CCUGCUGg- -3' miRNA: 3'- gaCAGCGUUGaCGCCAcGGACGAUgu -5' |
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| 7243 | 5' | -55.3 | NC_001900.1 | + | 2673 | 1.09 | 0.000686 |
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Target: 5'- aCUGUCGCAACUGCGGUGCCUGCUACAa -3' miRNA: 3'- -GACAGCGUUGACGCCACGGACGAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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